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• W196777009 abstract "Research Article1 September 1990free access Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction. A. Gallusser A. Gallusser Microbiology Department, University of Basel, Switzerland. Search for more papers by this author A. Kuhn A. Kuhn Microbiology Department, University of Basel, Switzerland. Search for more papers by this author A. Gallusser A. Gallusser Microbiology Department, University of Basel, Switzerland. Search for more papers by this author A. Kuhn A. Kuhn Microbiology Department, University of Basel, Switzerland. Search for more papers by this author Author Information A. Gallusser1 and A. Kuhn1 1Microbiology Department, University of Basel, Switzerland. The EMBO Journal (1990)9:2723-2729https://doi.org/10.1002/j.1460-2075.1990.tb07459.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Bacteriophage M13 procoat protein is synthesized on free polysomes prior to its assembly into the inner membrane of Escherichia coli. As an initial step of the membrane insertion pathway, the precursor protein interacts with the cytoplasmic face of the inner membrane. We have used oligonucleotide-directed mutagenesis to study the regions of the procoat protein involved in membrane binding. We find that there is an absolute requirement for positively charged amino acids at both ends of the protein. Replacing these with negatively charged residues resulted in an accumulation of the precursor in the cytoplasm. We propose that the positively charged amino acids are directly involved in membrane binding, possibly directly to the negatively charged phospholipid head groups. This was tested in vitro with artificial liposomes. Whereas wild-type procoat interacted with these liposomes, we found that procoat mutants with negatively charged amino acids at both ends did not bind. Therefore, we conclude that newly synthesized M13 procoat protein binds electrostatically to the negatively charged inner membrane of E. coli. Previous ArticleNext Article Volume 9Issue 91 September 1990In this issue RelatedDetailsLoading ..." @default.
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• W196777009 title "Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction." @default.
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• W196777009 doi "https://doi.org/10.1002/j.1460-2075.1990.tb07459.x" @default.
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