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- W1967778810 abstract "We report on the development and application of a smart activatable peptide-based probe for detection of Ras-related farnesyl protein transferase (FPT). Upon farnesylation by FPT, the probe was brought close to a hydrophobic milieu and as a consequence emitted fluorescent light that could be detected by several media, such as fluorescence microscopy, a plate reader, and an optical imaging system. A FPT activity assay confirmed the specificity of the probe (IC50 = 1.2 muM) for FPT compared to that of the native peptide (IC50 = 0.17 muM). In addition, the probe has remarkable binding constant, Kd = 26 nM. The specificity of enzyme activation was proved in pure enzyme assays as well as in cell-based assays. Furthermore, the fluorescent enhancement of the probe was 30-fold stronger than the control peptide in a live cell assay." @default.
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- W1967778810 date "2006-08-18" @default.
- W1967778810 modified "2023-09-25" @default.
- W1967778810 title "Imaging Farnesyl Protein Transferase Using a Topologically Activated Probe" @default.
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- W1967778810 doi "https://doi.org/10.1021/ja063599x" @default.
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