Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967793886> ?p ?o ?g. }
- W1967793886 endingPage "270" @default.
- W1967793886 startingPage "263" @default.
- W1967793886 abstract "Ribulose-1,5-bisphosphate carboxylase/oxygenase from spinach was inactivated by a carboxyl-directed reagent, Woodward's reagent K (WRK). The inactivation followed pseudo-first-order kinetics. The reaction order with respect to inactivation by WRK was 1.1, suggesting that inactivation was the consequence of modifying a single residue per active site. The substrate ribulose 1,5-bisphosphate (RBP), two competitive inhibitors, fructose 1,6-bisphosphate (FBP) and sedoheptulose 1,7-bisphosphate (SBP), and a number of sugars-phosphate protected against inactivation by WRK. SBP was a strong protector, displaying a dissociation constant (Kd) of 3 μm with native RBP carboxylase. Pretreatment of RBP carboxylase with diethyl pyrocarbonate prevented WRK incorporation into the enzyme. The enol ester derivative produced by reaction of WRK with RBP carboxylase has a maximal absorbance at 346 nm, and the extinction coefficient was found to be 12300 ± 700 m−1 cm−1. Spectrophotometric titration of the number of carboxyl groups modified by WRK in RBP carboxylase/oxygenase in the presence and in the absence of SBP suggests that inactivation was associated with the modification of one carboxyl group per active site." @default.
- W1967793886 created "2016-06-24" @default.
- W1967793886 creator A5079701047 @default.
- W1967793886 creator A5087007557 @default.
- W1967793886 date "1984-06-01" @default.
- W1967793886 modified "2023-10-15" @default.
- W1967793886 title "Modification of carboxyl groups at the active site of ribulose-1,5-bisphosphate carboxylase/oxygenase" @default.
- W1967793886 cites W1526571049 @default.
- W1967793886 cites W1532874244 @default.
- W1967793886 cites W1533618603 @default.
- W1967793886 cites W1536408663 @default.
- W1967793886 cites W1542418181 @default.
- W1967793886 cites W1550877969 @default.
- W1967793886 cites W1560174874 @default.
- W1967793886 cites W1604233325 @default.
- W1967793886 cites W1655141131 @default.
- W1967793886 cites W1963694848 @default.
- W1967793886 cites W1969016352 @default.
- W1967793886 cites W1973645653 @default.
- W1967793886 cites W1976566308 @default.
- W1967793886 cites W1978784439 @default.
- W1967793886 cites W1984010866 @default.
- W1967793886 cites W1989291578 @default.
- W1967793886 cites W1995592500 @default.
- W1967793886 cites W1998829663 @default.
- W1967793886 cites W1999629473 @default.
- W1967793886 cites W2004395803 @default.
- W1967793886 cites W2005200435 @default.
- W1967793886 cites W2006849276 @default.
- W1967793886 cites W2014336412 @default.
- W1967793886 cites W2016287008 @default.
- W1967793886 cites W2018025094 @default.
- W1967793886 cites W2021380124 @default.
- W1967793886 cites W2022510326 @default.
- W1967793886 cites W2030642428 @default.
- W1967793886 cites W2033086903 @default.
- W1967793886 cites W2043348980 @default.
- W1967793886 cites W2050438274 @default.
- W1967793886 cites W2050446537 @default.
- W1967793886 cites W2063936382 @default.
- W1967793886 cites W2072781284 @default.
- W1967793886 cites W2077173882 @default.
- W1967793886 cites W2079059245 @default.
- W1967793886 cites W2087926033 @default.
- W1967793886 cites W2089078037 @default.
- W1967793886 cites W2089188790 @default.
- W1967793886 cites W2111301002 @default.
- W1967793886 cites W2170986838 @default.
- W1967793886 cites W2222562979 @default.
- W1967793886 cites W275295038 @default.
- W1967793886 cites W333539686 @default.
- W1967793886 cites W91326892 @default.
- W1967793886 doi "https://doi.org/10.1016/0003-9861(84)90387-4" @default.
- W1967793886 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6587831" @default.
- W1967793886 hasPublicationYear "1984" @default.
- W1967793886 type Work @default.
- W1967793886 sameAs 1967793886 @default.
- W1967793886 citedByCount "8" @default.
- W1967793886 crossrefType "journal-article" @default.
- W1967793886 hasAuthorship W1967793886A5079701047 @default.
- W1967793886 hasAuthorship W1967793886A5087007557 @default.
- W1967793886 hasConcept C140137476 @default.
- W1967793886 hasConcept C161790260 @default.
- W1967793886 hasConcept C165474305 @default.
- W1967793886 hasConcept C178790620 @default.
- W1967793886 hasConcept C181199279 @default.
- W1967793886 hasConcept C185592680 @default.
- W1967793886 hasConcept C24428623 @default.
- W1967793886 hasConcept C2777414806 @default.
- W1967793886 hasConcept C2777824588 @default.
- W1967793886 hasConcept C2779966244 @default.
- W1967793886 hasConcept C2780054949 @default.
- W1967793886 hasConcept C40875361 @default.
- W1967793886 hasConcept C41183919 @default.
- W1967793886 hasConcept C50212798 @default.
- W1967793886 hasConcept C55493867 @default.
- W1967793886 hasConcept C71240020 @default.
- W1967793886 hasConceptScore W1967793886C140137476 @default.
- W1967793886 hasConceptScore W1967793886C161790260 @default.
- W1967793886 hasConceptScore W1967793886C165474305 @default.
- W1967793886 hasConceptScore W1967793886C178790620 @default.
- W1967793886 hasConceptScore W1967793886C181199279 @default.
- W1967793886 hasConceptScore W1967793886C185592680 @default.
- W1967793886 hasConceptScore W1967793886C24428623 @default.
- W1967793886 hasConceptScore W1967793886C2777414806 @default.
- W1967793886 hasConceptScore W1967793886C2777824588 @default.
- W1967793886 hasConceptScore W1967793886C2779966244 @default.
- W1967793886 hasConceptScore W1967793886C2780054949 @default.
- W1967793886 hasConceptScore W1967793886C40875361 @default.
- W1967793886 hasConceptScore W1967793886C41183919 @default.
- W1967793886 hasConceptScore W1967793886C50212798 @default.
- W1967793886 hasConceptScore W1967793886C55493867 @default.
- W1967793886 hasConceptScore W1967793886C71240020 @default.
- W1967793886 hasIssue "2" @default.
- W1967793886 hasLocation W19677938861 @default.
- W1967793886 hasLocation W19677938862 @default.
- W1967793886 hasOpenAccess W1967793886 @default.
- W1967793886 hasPrimaryLocation W19677938861 @default.