FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967814139> ?p ?o ?g. }

• W1967814139 endingPage "222" @default.
• W1967814139 startingPage "222" @default.
• W1967814139 abstract "One of the major issues in stem cell biology is to determine the conditions that enable stem cell culture, which has been slow and laborious due to the present nature of culture systems. Our current research is designed to leverage existing robotic and fluid handling technologies with the unique fluid control and microenvironment properties of the microscale, along with the extensive expertise in stem cell research. One key advantage of microfluidic systems for stem cell research is the ability to ultra-miniaturize the cell-based assays. Swine adipose-derived stem cells (ADSCs) were cultured and differentiated into adipogenic and osteogenic cells. A fluid handling robot was used to implement the passive pumping in the microdevices, which did not require any fluid connectors. A 192-channel micro-conduit polydimethylsiloxane (PDMS) array was made using soft lithography and bonded onto a standard microplate. The fluid handling robot was programmed to load cells and change medium. The total time to change medium for 192 channels was 30 min. Swine adipose-derived stem cells were cultured using DMEM + 10% fetal bovine serum (FBS) for six days in a 100% humidified 5% CO2 atmosphere. Two different cell concentrations were compared (1 � 106 cells and 2 � 106). The ADSCs were differentiated into adipogenic and osteogenic cells using specific differentiation media for the following ten days. The medium was changed every 24 h by the fluid handling robot. To assess for the differentiation, the adipogenic and osteogenic cells were stained using oil red O and alizarin red S to verify fat and calcium formation, respectively. Fatty acid accumulation was confirmed by red-stained lipid vesicles inside the adipogenic cells, and calcium formation was observed as red-stained calcium deposits around the osteogenic cells. We also determined that the concentration of 1 � 106 cells (equivalent to 1500 cells per channel) gave better results than the concentration of 2 � 106 cells (equivalent to 3000 cells per channel) in terms of cell morphology and differentiation parameters. The use of multifactorial directed differentiation using high-speed robotic systems, as employed in our research, will enable the examination of large matrices of culture and differentiation conditions for stem cells. Furthermore, our approach enables the use of gene expression analysis and other analytical methodologies to study the differentiation and cell function of cells cultured under essentially unlimited conditions. Using the automated microscale system in large factorial experiments allows analysis of the basic mechanisms underlying stem cell development in vitro, and ultimately in vivo. This research was supported by the Illinois Regenerative Medicine Institute (IRMI)." @default.
• W1967814139 created "2016-06-24" @default.
• W1967814139 creator A5009466330 @default.
• W1967814139 creator A5054537940 @default.
• W1967814139 creator A5062551393 @default.
• W1967814139 creator A5067298178 @default.
• W1967814139 creator A5079030399 @default.
• W1967814139 date "2008-01-01" @default.
• W1967814139 modified "2023-09-25" @default.
• W1967814139 title "284 HIGH-THROUGHPUT MICROFLUIDIC TECHNOLOGIES FOR STEM CELL RESEARCH" @default.
• W1967814139 doi "https://doi.org/10.1071/rdv20n1ab284" @default.
• W1967814139 hasPublicationYear "2008" @default.
• W1967814139 type Work @default.
• W1967814139 sameAs 1967814139 @default.
• W1967814139 citedByCount "0" @default.
• W1967814139 crossrefType "journal-article" @default.
• W1967814139 hasAuthorship W1967814139A5009466330 @default.
• W1967814139 hasAuthorship W1967814139A5054537940 @default.
• W1967814139 hasAuthorship W1967814139A5062551393 @default.
• W1967814139 hasAuthorship W1967814139A5067298178 @default.
• W1967814139 hasAuthorship W1967814139A5079030399 @default.
• W1967814139 hasConcept C122927707 @default.
• W1967814139 hasConcept C127413603 @default.
• W1967814139 hasConcept C136229726 @default.
• W1967814139 hasConcept C1491633281 @default.
• W1967814139 hasConcept C171089720 @default.
• W1967814139 hasConcept C171250308 @default.
• W1967814139 hasConcept C185592680 @default.
• W1967814139 hasConcept C192562407 @default.
• W1967814139 hasConcept C198826908 @default.
• W1967814139 hasConcept C2779404806 @default.
• W1967814139 hasConcept C28328180 @default.
• W1967814139 hasConcept C55493867 @default.
• W1967814139 hasConcept C8673954 @default.
• W1967814139 hasConcept C86803240 @default.
• W1967814139 hasConcept C95444343 @default.
• W1967814139 hasConceptScore W1967814139C122927707 @default.
• W1967814139 hasConceptScore W1967814139C127413603 @default.
• W1967814139 hasConceptScore W1967814139C136229726 @default.
• W1967814139 hasConceptScore W1967814139C1491633281 @default.
• W1967814139 hasConceptScore W1967814139C171089720 @default.
• W1967814139 hasConceptScore W1967814139C171250308 @default.
• W1967814139 hasConceptScore W1967814139C185592680 @default.
• W1967814139 hasConceptScore W1967814139C192562407 @default.
• W1967814139 hasConceptScore W1967814139C198826908 @default.
• W1967814139 hasConceptScore W1967814139C2779404806 @default.
• W1967814139 hasConceptScore W1967814139C28328180 @default.
• W1967814139 hasConceptScore W1967814139C55493867 @default.
• W1967814139 hasConceptScore W1967814139C8673954 @default.
• W1967814139 hasConceptScore W1967814139C86803240 @default.
• W1967814139 hasConceptScore W1967814139C95444343 @default.
• W1967814139 hasIssue "1" @default.
• W1967814139 hasLocation W19678141391 @default.
• W1967814139 hasOpenAccess W1967814139 @default.
• W1967814139 hasPrimaryLocation W19678141391 @default.
• W1967814139 hasRelatedWork W1970304548 @default.
• W1967814139 hasRelatedWork W1985527854 @default.
• W1967814139 hasRelatedWork W2005270887 @default.
• W1967814139 hasRelatedWork W2350665201 @default.
• W1967814139 hasRelatedWork W2397997941 @default.
• W1967814139 hasRelatedWork W2612337719 @default.
• W1967814139 hasRelatedWork W2941398497 @default.
• W1967814139 hasRelatedWork W3007636232 @default.
• W1967814139 hasRelatedWork W3012664368 @default.
• W1967814139 hasRelatedWork W3203553651 @default.
• W1967814139 hasVolume "20" @default.
• W1967814139 isParatext "false" @default.
• W1967814139 isRetracted "false" @default.
• W1967814139 magId "1967814139" @default.
• W1967814139 workType "article" @default.