Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967862247> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1967862247 endingPage "998" @default.
- W1967862247 startingPage "990" @default.
- W1967862247 abstract "Monoclonal antibody 4B1 binds to a conformational epitope on the periplasmic surface of the lactose permease of Escherichia coli, uncoupling lactose and H+ translocation in a manner indicating that it blocks deprotonation [Carrasco, N., Viitanen, P., Herzlinger, D., & Kaback, H. R. (1984) Biochemistry 23, 3681; Herzlinger, D., Viitanen, P., Carrasco, N., & Kaback, H. R. (1984) Biochemistry 23, 3688]. In this paper, 4B1 binding to purified lactose permease is shown to exhibit a KD of about 5 × 10-10 M by surface plasmon resonance. Furthermore, the combined use of mutants containing 6 contiguous His residues in each periplasmic loop in the permease and Cys-scanning mutagenesis in conjunction with chemical labeling demonstrates that 4B1 binds specifically to the periplasmic loop between helices VII and VIII and that Phe247 and Gly254 are the primary determinants. Remarkably, although 4B1 binding uncouples lactose and H+ translocation, none of the amino acid residues in periplasmic loops, particularly Phe247 or Gly254, play an important role in the transport mechanism. Moreover, binding of avidin to biotinylated Glu255→Cys in the loop containing the epitope has no effect on transport activity. Therefore, the uncoupling effect of 4B1 involves highly specific interactions which in all likelihood exert a torsional effect on the loop, resulting in a conformational change in helix VII and/or VIII that alters the pKas of residues involved in lactose-coupled H+ translocation." @default.
- W1967862247 created "2016-06-24" @default.
- W1967862247 creator A5013090368 @default.
- W1967862247 creator A5031873929 @default.
- W1967862247 creator A5051144285 @default.
- W1967862247 creator A5067934090 @default.
- W1967862247 date "1996-01-01" @default.
- W1967862247 modified "2023-09-23" @default.
- W1967862247 title "Identification of the Epitope for Monoclonal Antibody 4B1 Which Uncouples Lactose and Proton Translocation in the Lactose Permease of <i>Escherichia </i><i>coli</i>" @default.
- W1967862247 cites W1015595619 @default.
- W1967862247 cites W1533468568 @default.
- W1967862247 cites W1602945734 @default.
- W1967862247 cites W1606178002 @default.
- W1967862247 cites W1923710818 @default.
- W1967862247 cites W2053721311 @default.
- W1967862247 cites W2102131116 @default.
- W1967862247 doi "https://doi.org/10.1021/bi952166w" @default.
- W1967862247 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8547282" @default.
- W1967862247 hasPublicationYear "1996" @default.
- W1967862247 type Work @default.
- W1967862247 sameAs 1967862247 @default.
- W1967862247 citedByCount "60" @default.
- W1967862247 countsByYear W19678622472012 @default.
- W1967862247 countsByYear W19678622472013 @default.
- W1967862247 countsByYear W19678622472014 @default.
- W1967862247 countsByYear W19678622472015 @default.
- W1967862247 countsByYear W19678622472017 @default.
- W1967862247 countsByYear W19678622472019 @default.
- W1967862247 countsByYear W19678622472020 @default.
- W1967862247 countsByYear W19678622472021 @default.
- W1967862247 crossrefType "journal-article" @default.
- W1967862247 hasAuthorship W1967862247A5013090368 @default.
- W1967862247 hasAuthorship W1967862247A5031873929 @default.
- W1967862247 hasAuthorship W1967862247A5051144285 @default.
- W1967862247 hasAuthorship W1967862247A5067934090 @default.
- W1967862247 hasConcept C104317684 @default.
- W1967862247 hasConcept C159654299 @default.
- W1967862247 hasConcept C185592680 @default.
- W1967862247 hasConcept C190114821 @default.
- W1967862247 hasConcept C195616568 @default.
- W1967862247 hasConcept C201663137 @default.
- W1967862247 hasConcept C203014093 @default.
- W1967862247 hasConcept C2778724459 @default.
- W1967862247 hasConcept C547475151 @default.
- W1967862247 hasConcept C55493867 @default.
- W1967862247 hasConcept C58598135 @default.
- W1967862247 hasConcept C86803240 @default.
- W1967862247 hasConceptScore W1967862247C104317684 @default.
- W1967862247 hasConceptScore W1967862247C159654299 @default.
- W1967862247 hasConceptScore W1967862247C185592680 @default.
- W1967862247 hasConceptScore W1967862247C190114821 @default.
- W1967862247 hasConceptScore W1967862247C195616568 @default.
- W1967862247 hasConceptScore W1967862247C201663137 @default.
- W1967862247 hasConceptScore W1967862247C203014093 @default.
- W1967862247 hasConceptScore W1967862247C2778724459 @default.
- W1967862247 hasConceptScore W1967862247C547475151 @default.
- W1967862247 hasConceptScore W1967862247C55493867 @default.
- W1967862247 hasConceptScore W1967862247C58598135 @default.
- W1967862247 hasConceptScore W1967862247C86803240 @default.
- W1967862247 hasIssue "3" @default.
- W1967862247 hasLocation W19678622471 @default.
- W1967862247 hasLocation W19678622472 @default.
- W1967862247 hasOpenAccess W1967862247 @default.
- W1967862247 hasPrimaryLocation W19678622471 @default.
- W1967862247 hasRelatedWork W1920853577 @default.
- W1967862247 hasRelatedWork W2002073309 @default.
- W1967862247 hasRelatedWork W2022959281 @default.
- W1967862247 hasRelatedWork W2035867956 @default.
- W1967862247 hasRelatedWork W2053756295 @default.
- W1967862247 hasRelatedWork W2167385584 @default.
- W1967862247 hasRelatedWork W2292519793 @default.
- W1967862247 hasRelatedWork W2314001747 @default.
- W1967862247 hasRelatedWork W4206287474 @default.
- W1967862247 hasRelatedWork W2430749155 @default.
- W1967862247 hasVolume "35" @default.
- W1967862247 isParatext "false" @default.
- W1967862247 isRetracted "false" @default.
- W1967862247 magId "1967862247" @default.
- W1967862247 workType "article" @default.