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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967868800> ?p ?o ?g. }

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• W1967868800 abstract "The modified nucleoside 1-methyladenosine (m 1 A) is found at position 58 in the TΨC loop of many eukaryotic tRNAs. The absence of m 1 A from all tRNAs in Saccharomyces cerevisiae mutants lacking Gcd10p elicits severe defects in processing and stability of initiator methionine tRNA (tRNA i Met ). Gcd10p is found in a complex with Gcd14p, which contains conserved motifs for binding S -adenosylmethionine (AdoMet). These facts, plus our demonstration that gcd14Δ cells lacked m 1 A, strongly suggested that Gcd10p/Gcd14p complex is the yeast tRNA(m 1 A)methyltransferase [(m 1 A)MTase]. Supporting this prediction, affinity-purified Gcd10p/Gcd14p complexes used AdoMet as a methyl donor to synthesize m 1 A in either total tRNA or purified tRNA i Met lacking only this modification. Kinetic analysis of the purified complex revealed K M values for AdoMet or tRNA i Met of 5.0 μM and 2.5 nM, respectively. Mutations in the predicted AdoMet-binding domain destroyed GCD14 function in vivo and (m 1 A)MTase activity in vitro . Purified Flag-tagged Gcd14p alone had no enzymatic activity and was severely impaired for tRNA-binding compared with the wild-type complex, suggesting that Gcd10p is required for tight binding of the tRNA substrate. Our results provide a demonstration of a two-component tRNA MTase and suggest that binding of AdoMet and tRNA substrates depends on different subunits of the complex." @default.
• W1967868800 created "2016-06-24" @default.
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• W1967868800 date "2000-04-25" @default.
• W1967868800 modified "2023-10-03" @default.
• W1967868800 title "The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of <i>Saccharomyces cerevisiae</i>" @default.
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• W1967868800 doi "https://doi.org/10.1073/pnas.090102597" @default.
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