FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967883399> ?p ?o ?g. }

• W1967883399 endingPage "e100543" @default.
• W1967883399 startingPage "e100543" @default.
• W1967883399 abstract "B. cereus possesses flagella which allow the organism to migrate within the eye during a blinding form of intraocular infection called endophthalmitis. Because flagella is a ligand for Toll-like receptor 5 (TLR5), we hypothesized that TLR5 contributed to endophthalmitis pathogenesis. Endophthalmitis was induced in C57BL/6J and TLR5−/− mice by injecting 100 CFU of B. cereus into the mid-vitreous. Eyes were analyzed for intraocular bacterial growth, retinal function, and inflammation by published methods. Purified B. cereus flagellin was also injected into the mid-vitreous of wild type C57BL/6J mice and inflammation was analyzed. TLR5 activation by B. cereus flagellin was also analyzed in vitro. B. cereus grew rapidly and at similar rates in infected eyes of C57BL/6J and TLR5−/− mice. A significant loss in retinal function in both groups of mice was observed at 8 and 12 hours postinfection. Retinal architecture disruption and acute inflammation (neutrophil infiltration and proinflammatory cytokine concentrations) increased and were significant at 8 and 12 hours postinfection. Acute inflammation was comparable in TLR5−/− and C57BL/6J mice. Physiological concentrations of purified B. cereus flagellin caused significant inflammation in C57BL/6J mouse eyes, but not to the extent of that observed during active infection. Purified B. cereus flagellin was a weak agonist for TLR5 in vitro. These results demonstrated that the absence of TLR5 did not have a significant effect on the evolution of B. cereus endophthalmitis. This disparity may be due to sequence differences in important TLR5 binding domains in B. cereus flagellin or the lack of flagellin monomers in the eye to activate TLR5 during infection. Taken together, these results suggest a limited role for flagellin/TLR5 interactions in B. cereus endophthalmitis. Based on this and previous data, the importance of flagella in this disease lies in its contribution to the motility of the organism within the eye during infection." @default.
• W1967883399 created "2016-06-24" @default.
• W1967883399 creator A5000532678 @default.
• W1967883399 creator A5017346259 @default.
• W1967883399 creator A5090065464 @default.
• W1967883399 date "2014-06-24" @default.
• W1967883399 modified "2023-09-23" @default.
• W1967883399 title "Role of TLR5 and Flagella in Bacillus Intraocular Infection" @default.
• W1967883399 cites W1517989718 @default.
• W1967883399 cites W1519633296 @default.
• W1967883399 cites W1615829672 @default.
• W1967883399 cites W1666110846 @default.
• W1967883399 cites W1801519214 @default.
• W1967883399 cites W1860199759 @default.
• W1967883399 cites W1900975454 @default.
• W1967883399 cites W1915024169 @default.
• W1967883399 cites W1929816290 @default.
• W1967883399 cites W1951255707 @default.
• W1967883399 cites W1968026360 @default.
• W1967883399 cites W1978980453 @default.
• W1967883399 cites W1983007329 @default.
• W1967883399 cites W1985185645 @default.
• W1967883399 cites W1987186922 @default.
• W1967883399 cites W1990834447 @default.
• W1967883399 cites W1993434371 @default.
• W1967883399 cites W1995401406 @default.
• W1967883399 cites W1996111152 @default.
• W1967883399 cites W2013583403 @default.
• W1967883399 cites W2016277483 @default.
• W1967883399 cites W2020040553 @default.
• W1967883399 cites W2020922034 @default.
• W1967883399 cites W2022156312 @default.
• W1967883399 cites W2022691304 @default.
• W1967883399 cites W2030627731 @default.
• W1967883399 cites W2033766416 @default.
• W1967883399 cites W2033833740 @default.
• W1967883399 cites W2038679047 @default.
• W1967883399 cites W2042711493 @default.
• W1967883399 cites W2045797529 @default.
• W1967883399 cites W2047329666 @default.
• W1967883399 cites W2047694702 @default.
• W1967883399 cites W2048285080 @default.
• W1967883399 cites W2052278197 @default.
• W1967883399 cites W2063677134 @default.
• W1967883399 cites W2063748502 @default.
• W1967883399 cites W2065876844 @default.
• W1967883399 cites W2071061970 @default.
• W1967883399 cites W2075806846 @default.
• W1967883399 cites W2075948284 @default.
• W1967883399 cites W2078064634 @default.
• W1967883399 cites W2078279139 @default.
• W1967883399 cites W2088232877 @default.
• W1967883399 cites W2093577783 @default.
• W1967883399 cites W2098006866 @default.
• W1967883399 cites W2101901168 @default.
• W1967883399 cites W2106882534 @default.
• W1967883399 cites W2109226264 @default.
• W1967883399 cites W2113760809 @default.
• W1967883399 cites W2117560359 @default.
• W1967883399 cites W2127336616 @default.
• W1967883399 cites W2130694284 @default.
• W1967883399 cites W2132658744 @default.
• W1967883399 cites W2133145885 @default.
• W1967883399 cites W2134270631 @default.
• W1967883399 cites W2134308642 @default.
• W1967883399 cites W2140193468 @default.
• W1967883399 cites W2142992860 @default.
• W1967883399 cites W2144730393 @default.
• W1967883399 cites W2151461473 @default.
• W1967883399 cites W2153517908 @default.
• W1967883399 cites W2154883084 @default.
• W1967883399 cites W2156212552 @default.
• W1967883399 cites W2157207084 @default.
• W1967883399 cites W2162904046 @default.
• W1967883399 cites W2167849015 @default.
• W1967883399 cites W4236130066 @default.
• W1967883399 doi "https://doi.org/10.1371/journal.pone.0100543" @default.
• W1967883399 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4068998" @default.
• W1967883399 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24959742" @default.
• W1967883399 hasPublicationYear "2014" @default.
• W1967883399 type Work @default.
• W1967883399 sameAs 1967883399 @default.
• W1967883399 citedByCount "30" @default.
• W1967883399 countsByYear W19678833992015 @default.
• W1967883399 countsByYear W19678833992016 @default.
• W1967883399 countsByYear W19678833992017 @default.
• W1967883399 countsByYear W19678833992018 @default.
• W1967883399 countsByYear W19678833992019 @default.
• W1967883399 countsByYear W19678833992020 @default.
• W1967883399 countsByYear W19678833992021 @default.
• W1967883399 countsByYear W19678833992022 @default.
• W1967883399 crossrefType "journal-article" @default.
• W1967883399 hasAuthorship W1967883399A5000532678 @default.
• W1967883399 hasAuthorship W1967883399A5017346259 @default.
• W1967883399 hasAuthorship W1967883399A5090065464 @default.
• W1967883399 hasBestOaLocation W19678833991 @default.
• W1967883399 hasConcept C164027704 @default.
• W1967883399 hasConcept C170493617 @default.

• next