Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967901726> ?p ?o ?g. }
- W1967901726 endingPage "110" @default.
- W1967901726 startingPage "94" @default.
- W1967901726 abstract "To investigate the physical and kinetic properties of sperm carnitine acetyltransferase, the enzyme was purified from bovine spermatozoa and heart muscle. Carnitine acetyltransferase was purified 580-fold from ejaculated bovine spermatozoa to a specific activity of 85 units/mg protein (95% homogeneity). Sperm carnitine acetyltransferase was characterized as a single polypeptide of Mr 62,000 and pI 8.2. Heart carnitine acetyltransferase was purified 650-fold by the same procedure to a final specific activity of 71 units/mg protein. The kinetic properties of purified bovine sperm carnitine acetyltransferase were consistent with the proposed function of this enzyme in acetylcarnitine pool formation. Product inhibition by either acetyl-l-carnitine or CoASH was not sufficient to predict significant in vivo inhibition of acetyl transfer. At high concentrations of l-carnitine, bovine sperm and heart carnitine acetyltransferases were most active with propionyl- and butyryl-CoA substrates, although octanoyl-, iso-butyryl-, and iso-valeryl-CoA were acceptable substrates. Binding of one substrate was enhanced by the presence of the second substrate. Carnitine analogs that have significance in reproduction, such as phosphorylcholine and taurine, did not inhibit carnitine acetyltransferase. Bovine sperm and heart carnitine acetyltransferases were indistinguishable on the basis of purification behavior, pI, pH optima, kinetic properties, acyl-CoA specificity, and sensitivity to sulfhydryl reagents and divalent cations; thus there was no indication that bovine sperm carnitine acetyltransferase is a sperm-specific isozyme." @default.
- W1967901726 created "2016-06-24" @default.
- W1967901726 creator A5002200455 @default.
- W1967901726 creator A5089539874 @default.
- W1967901726 date "1983-10-01" @default.
- W1967901726 modified "2023-09-23" @default.
- W1967901726 title "Purification and properties of carnitine acetyltransferases from bovine spermatozoa and heart" @default.
- W1967901726 cites W1485250221 @default.
- W1967901726 cites W148691387 @default.
- W1967901726 cites W1519674605 @default.
- W1967901726 cites W1531564348 @default.
- W1967901726 cites W1542788366 @default.
- W1967901726 cites W1546169749 @default.
- W1967901726 cites W1558548227 @default.
- W1967901726 cites W1596676075 @default.
- W1967901726 cites W1613234551 @default.
- W1967901726 cites W1625660792 @default.
- W1967901726 cites W1762929468 @default.
- W1967901726 cites W1810982715 @default.
- W1967901726 cites W1862213828 @default.
- W1967901726 cites W1865315855 @default.
- W1967901726 cites W1867252638 @default.
- W1967901726 cites W1870178267 @default.
- W1967901726 cites W1952321278 @default.
- W1967901726 cites W1963794212 @default.
- W1967901726 cites W1977066599 @default.
- W1967901726 cites W1991407793 @default.
- W1967901726 cites W2006884067 @default.
- W1967901726 cites W2014705327 @default.
- W1967901726 cites W2018217937 @default.
- W1967901726 cites W2024880921 @default.
- W1967901726 cites W2030492847 @default.
- W1967901726 cites W2032718183 @default.
- W1967901726 cites W2048990670 @default.
- W1967901726 cites W2054979333 @default.
- W1967901726 cites W2060702666 @default.
- W1967901726 cites W2065630232 @default.
- W1967901726 cites W2087821869 @default.
- W1967901726 cites W2092630364 @default.
- W1967901726 cites W2101677404 @default.
- W1967901726 cites W2106541035 @default.
- W1967901726 cites W2108310592 @default.
- W1967901726 cites W2126100793 @default.
- W1967901726 cites W2134635730 @default.
- W1967901726 cites W2144622331 @default.
- W1967901726 cites W2155570544 @default.
- W1967901726 cites W2212175354 @default.
- W1967901726 cites W2426921010 @default.
- W1967901726 cites W2436228180 @default.
- W1967901726 cites W4254807231 @default.
- W1967901726 cites W4293247451 @default.
- W1967901726 cites W70241245 @default.
- W1967901726 cites W93351162 @default.
- W1967901726 cites W1975762197 @default.
- W1967901726 doi "https://doi.org/10.1016/0003-9861(83)90274-6" @default.
- W1967901726 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6639064" @default.
- W1967901726 hasPublicationYear "1983" @default.
- W1967901726 type Work @default.
- W1967901726 sameAs 1967901726 @default.
- W1967901726 citedByCount "13" @default.
- W1967901726 countsByYear W19679017262013 @default.
- W1967901726 countsByYear W19679017262014 @default.
- W1967901726 countsByYear W19679017262019 @default.
- W1967901726 countsByYear W19679017262021 @default.
- W1967901726 crossrefType "journal-article" @default.
- W1967901726 hasAuthorship W1967901726A5002200455 @default.
- W1967901726 hasAuthorship W1967901726A5089539874 @default.
- W1967901726 hasConcept C104317684 @default.
- W1967901726 hasConcept C119157956 @default.
- W1967901726 hasConcept C134651460 @default.
- W1967901726 hasConcept C181199279 @default.
- W1967901726 hasConcept C185592680 @default.
- W1967901726 hasConcept C2777553358 @default.
- W1967901726 hasConcept C2778435403 @default.
- W1967901726 hasConcept C2779123261 @default.
- W1967901726 hasConcept C2779649592 @default.
- W1967901726 hasConcept C2781087480 @default.
- W1967901726 hasConcept C2910274206 @default.
- W1967901726 hasConcept C55493867 @default.
- W1967901726 hasConcept C59822182 @default.
- W1967901726 hasConcept C86803240 @default.
- W1967901726 hasConceptScore W1967901726C104317684 @default.
- W1967901726 hasConceptScore W1967901726C119157956 @default.
- W1967901726 hasConceptScore W1967901726C134651460 @default.
- W1967901726 hasConceptScore W1967901726C181199279 @default.
- W1967901726 hasConceptScore W1967901726C185592680 @default.
- W1967901726 hasConceptScore W1967901726C2777553358 @default.
- W1967901726 hasConceptScore W1967901726C2778435403 @default.
- W1967901726 hasConceptScore W1967901726C2779123261 @default.
- W1967901726 hasConceptScore W1967901726C2779649592 @default.
- W1967901726 hasConceptScore W1967901726C2781087480 @default.
- W1967901726 hasConceptScore W1967901726C2910274206 @default.
- W1967901726 hasConceptScore W1967901726C55493867 @default.
- W1967901726 hasConceptScore W1967901726C59822182 @default.
- W1967901726 hasConceptScore W1967901726C86803240 @default.
- W1967901726 hasIssue "1" @default.
- W1967901726 hasLocation W19679017261 @default.
- W1967901726 hasLocation W19679017262 @default.