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- W1967939130 abstract "The aim of this investigation was to study the influence of freezing and thawing of semen on motility, penetration ability in cervical mucus and fertilizing capacity of spermatozoa. A total of 65 ejaculates was frozen and thawed. 50 specimens came from 7 heterologous insemination donors and 15 from homologous donors. Fresh ejaculates were studied and frozen immediately. After dilution in a progective medium the mixture was preserved in sealed plastic straws and cooled in liquid nitrogen. Storage time varied from 1 day to 5 weeks. Thawing was done in a water bath at 37 degrees C. The postthaw, otility, penetration ability and duration of motility in cervical mucus of spermatozoa were reduced by the freeze-preservation procedure. However in some of the samples spermatozoa had an unchanged penetration ability of cervical mucus. The women who were inseminated belonged to the sterility groups of the clinic but were apparently were performed in 14 women and 15 homologous inseminations in 4 women. No conceptions occurred after using semen preserved at 196 degrees C. Later the 14 women who had received heterologous insemination with freeze preserved semen were inseminated with fresh semen from the same donors and 8 conceptions occurred. From these results and reports by others it is concluded that postthaw mobility is a poor prediction of fertility. For fresh donor semen there is a significant correlation between conception rate and furation of sperm motility in postthaw specimens may explain in part the reduced fertility. Sperm density of the sample influenced motility recovery. Semen samples of law sperm density are not suitable for freeze preserving. Undetected damage to spermatozoa during freezing and thawing is thought to cause the discrepancy between fertility and the results of the tests. The fertilizing capacity of freeze-preserved semen was more reduced than expected." @default.
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- W1967939130 date "1973-01-01" @default.
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- W1967939130 title "Fertility, Motility and Penetration in Cervical Mucus of Freeze-Preserved Human Spermatozoa" @default.
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- W1967939130 doi "https://doi.org/10.3109/00016347309158316" @default.
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