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• W1967947582 abstract "Background: In the present study, the resistance mechanisms against carbapenems and aminoglyco- sides for 23 strains of multi-drug-resistant Acineto- bacter baumannii isolated at a university hospital were investigated. Methods: The minimal inhibitory concentrations (MICs) were determined via broth microdilution or Etest. The genes encoding OXA-type carbapenemases and 16S rRNA methylase were identified using multiplex PCR, and the amplified products were sequenced. Conju- gation experiments were conducted, and an epi- demiologic study was performed using enterobac- terial repetitive intergenic consensus (ERIC)-PCR. Results: In the isolates, the MICs of the tested ami- noglycosides, including arbekacin, were >1024 μg/ mL; the MICs of aztreonam, cefepime, ceftazidime, and ciprofloxacin ranged from 64 to 128 μg/mL; and the MICs of carbapenem ranged from 32 to 64 μg/ mL, as determined through the broth microdilution test. According to the E-test, the MICs of ampicillin/ sulbactam and colistin were 8 and 0.25 to 0.38 μg/ mL, respectively. Sequence analysis confirmed that all of the isolates expressed carbapenemases OXA- 23 and OXA-66, as well as armA 16S rRNA methy- lase. In addition, ISAba1 was identified upstream of the gene encoding OXA-23. OXA-23 and armA were not transferred to Escherichia coli J53 cells in the transconjugation experiments. ERIC-PCR molecular fingerprinting produced a single pattern in all cases. Conclusion: The co-production of OXA-23 and armA 16S rRNA methylase may be attributed to the multi- drug resistance of the A. baumannii isolates in the present study. Stricter surveillance and more rapid detection are necessary to prevent the spread of this type of resistance in the future. (Korean J Clin Microbiol 2011;14:67-73)" @default.
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• W1967947582 date "2011-01-01" @default.
• W1967947582 modified "2023-09-26" @default.
• W1967947582 title "Characterization ofAcinetobacter baumanniiCo-producing Carbapenemases OXA-23 and OXA-66, andarmA16S Ribosomal RNA Methylase at a University Hospital in South Korea" @default.
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• W1967947582 doi "https://doi.org/10.5145/kjcm.2011.14.2.67" @default.
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