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- W1967956098 abstract "We developed an enzyme immunoassay (EIA) for bovine GH (bGH) which is based on indirect competitive immunoassay in culture medium from a bovine pituitary cell culture. 40μl cell culture samples (or bGH standard) and bGH antibody (rabbit anti-bGH) were added to the 96 well microplate coated with secondary antibody (Goat anti-rabbit IgG), and incubated for 24h at 37°C. Biotin-label bGH was added and incubated further for 24h at 37°C, and biotinylated bGH was linked with streptoavidin-peroxidase. Substrates for peroxidase were added to the plate and incubated for 1h at 4°C. The enzyme reaction was stopped with 4N H2SO4, and the absorbency at 450nm was measured with an ELISA Reader. The coefficients of intra-assay and inter-assay variations were 4.13-7.59% and 3.71-8.27%, respectively. The regression equation and correlation coefficients with the radioimmunoassay (RIA) were y(RIA)=1.9986×(ETA)-1.3921 and 0.9701(n=27), respectively. Collectively, the present assay provides a reliable alternative to RIA and offers the major advantage of eliminating radioactive reagents and counting equipment." @default.
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- W1967956098 date "1997-01-01" @default.
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- W1967956098 title "Competitive Enzyme Immunoassay for Bovine Growth Hormone." @default.
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- W1967956098 doi "https://doi.org/10.1507/endocrj.44.195" @default.
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