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- W1967987132 abstract "Abstract Objective To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro. Methods The plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM. Results Fusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction. Conclusion These findings represent a novel strategy in EPC transplantation treatment for vessel injury." @default.
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- W1967987132 date "2011-08-01" @default.
- W1967987132 modified "2023-09-26" @default.
- W1967987132 title "A novel vWF A3-GPI modified EPCs to enhance its adhesion ability to collegen" @default.
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- W1967987132 doi "https://doi.org/10.1016/s1000-1948(11)60036-1" @default.
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