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- W1968042616 abstract "The ability to attach firmly chelated metal ions or powerful chelating agents to sites on biological molecules can enhance the utility of a number of physical techniques now used in the study of biological systems. A “bifunctional” chelating agent, containing both an EDTA group and a diazonium group, has been prepared and coupled to human serum albumin. The extent of labeling under various conditions and the amino-acid sidechains labeled have been investigated. The reaction of protein-bound chelating groups with added metal ions has been studied, with the finding that only about 40–50% of these groups are available to bind metal ions. Proteolysis of the products leads to recovery of full metal-binding capacity. Properties of the products in vivo are discussed." @default.
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- W1968042616 date "1978-11-01" @default.
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- W1968042616 title "The attachment of metal-chelating groups to proteins: Tagging of albumin by diazonium coupling and use of the products as radiopharmaceuticals" @default.
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- W1968042616 doi "https://doi.org/10.1016/0020-708x(78)90107-2" @default.
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