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- W1968183789 abstract "The budding yeast, Saccharomyces cerevisiae, responds to changes in external osmolarity through the activation of an osmosensing signal transduction pathway. Using lacZ-reporter gene fusions, clonal cell lines were screened for levels of beta-galactosidase activity in the presence or absence of osmotic stress. A screen of 9,000 transformants displayed 663 (7%) gene fusions that were active in rich medium. Each of the transformants were also assayed for gene activity 24 h following a transfer to high osmolarity medium (0.6 M NaCl) and of the 9,000 clonal cell lines, 86 (1%) displayed a decrease in expression, and seven (0.1%) displayed a reproducible increase in gene expression during primary screening. The chromosomal loci of the lacZ insertions were determined, and the gene(s) associated with that site was examined for osmotically induced expression using RNA blot analysis. Five stress-activated genes were analysed by RNA blot: YDL222C, NMD2, PTC7, FAA4 and YRF1. The genes identified by this screen encompass cellular adaptations to stress including signal transduction, protein myristoylation and fatty acid/sphingolipid content in the cell membrane." @default.
- W1968183789 created "2016-06-24" @default.
- W1968183789 creator A5058478601 @default.
- W1968183789 creator A5070391911 @default.
- W1968183789 date "2003-01-01" @default.
- W1968183789 modified "2023-09-28" @default.
- W1968183789 title "A genetic screen for yeast genes induced by sustained osmotic stress" @default.
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- W1968183789 doi "https://doi.org/10.1002/yea.1019" @default.
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