FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968206876> ?p ?o ?g. }

• W1968206876 endingPage "247" @default.
• W1968206876 startingPage "238" @default.
• W1968206876 abstract "Tracing cell movements in vivo yields important clues to organogenesis, yet it has been challenging to accurately and reproducibly fluorescently mark single and small groups of cells to build a picture of tissue assembly. In the early embryo, the small size (hundreds of cells) of progenitor cell regions has made it easier to identify and selectively mark superficially located cells by glass needle injection. However, during early organogenesis, subregions of interest may be several millions of cells in volume located deeper within the embryo requiring an alternative approach. Here, we combined (confocal and 2-photon) photoactivation cell labeling and multi-position, multi-time imaging to trace single cell and small subgroups of cells in the developing brain and spinal cord. We compared the photostability and photoefficiency of a photoswitchable fluorescent protein, PSCFP2, with a novel nuclear localized H2B-PSCFP2 protein. We showed that both fluorescent proteins have similar photophysical properties and H2B-PSCFP2 is more effective in single cell identification in dense tissue. To accurately and reproducibly fluorescently trace subregions of cells in a 3D tissue volume, we developed a protocol for multi-position photoactivation and multi-time acquisition in the chick spinal cord in up to 8 tissue sections. We applied our techniques to address the formation of the sympathetic ganglia, a major component of the autonomic nervous system, and showed there are phenotypic differences between early and later emerging neural crest cells and their positions in the developing ganglia. Thus, targeted fluorescent cell marking by confocal or 2-photon multi-position photoactivation and multi-time acquisition offer a more efficient, less invasive technique to trace cell movements in large regions of interest and move us closer towards mapping the cellular events of organogenesis." @default.
• W1968206876 created "2016-06-24" @default.
• W1968206876 creator A5001227194 @default.
• W1968206876 creator A5002169187 @default.
• W1968206876 creator A5010129042 @default.
• W1968206876 creator A5021609999 @default.
• W1968206876 creator A5032633750 @default.
• W1968206876 date "2009-10-01" @default.
• W1968206876 modified "2023-10-06" @default.
• W1968206876 title "Watching the assembly of an organ a single cell at a time using confocal multi-position photoactivation and multi-time acquisition" @default.
• W1968206876 cites W125412302 @default.
• W1968206876 cites W1494673328 @default.
• W1968206876 cites W1588332886 @default.
• W1968206876 cites W1931761759 @default.
• W1968206876 cites W1975443729 @default.
• W1968206876 cites W1992778213 @default.
• W1968206876 cites W1996657515 @default.
• W1968206876 cites W1998279433 @default.
• W1968206876 cites W2000299259 @default.
• W1968206876 cites W2003298106 @default.
• W1968206876 cites W2004102023 @default.
• W1968206876 cites W2007314180 @default.
• W1968206876 cites W2019612613 @default.
• W1968206876 cites W2020838908 @default.
• W1968206876 cites W2023650634 @default.
• W1968206876 cites W2027030154 @default.
• W1968206876 cites W2032756435 @default.
• W1968206876 cites W2038726927 @default.
• W1968206876 cites W2077765163 @default.
• W1968206876 cites W2080165381 @default.
• W1968206876 cites W2088864569 @default.
• W1968206876 cites W2090338864 @default.
• W1968206876 cites W2093030333 @default.
• W1968206876 cites W2104964229 @default.
• W1968206876 cites W2110292170 @default.
• W1968206876 cites W2120599929 @default.
• W1968206876 cites W2126878033 @default.
• W1968206876 cites W2135256472 @default.
• W1968206876 cites W2140034535 @default.
• W1968206876 cites W2140588274 @default.
• W1968206876 cites W2143701634 @default.
• W1968206876 cites W2170822244 @default.
• W1968206876 cites W46841966 @default.
• W1968206876 cites W63274132 @default.
• W1968206876 doi "https://doi.org/10.4161/org.5.4.10482" @default.
• W1968206876 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2878753" @default.
• W1968206876 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20539744" @default.
• W1968206876 hasPublicationYear "2009" @default.
• W1968206876 type Work @default.
• W1968206876 sameAs 1968206876 @default.
• W1968206876 citedByCount "11" @default.
• W1968206876 countsByYear W19682068762012 @default.
• W1968206876 countsByYear W19682068762013 @default.
• W1968206876 countsByYear W19682068762022 @default.
• W1968206876 crossrefType "journal-article" @default.
• W1968206876 hasAuthorship W1968206876A5001227194 @default.
• W1968206876 hasAuthorship W1968206876A5002169187 @default.
• W1968206876 hasAuthorship W1968206876A5010129042 @default.
• W1968206876 hasAuthorship W1968206876A5021609999 @default.
• W1968206876 hasAuthorship W1968206876A5032633750 @default.
• W1968206876 hasBestOaLocation W19682068762 @default.
• W1968206876 hasConcept C103378370 @default.
• W1968206876 hasConcept C104317684 @default.
• W1968206876 hasConcept C105702510 @default.
• W1968206876 hasConcept C113976765 @default.
• W1968206876 hasConcept C121332964 @default.
• W1968206876 hasConcept C126749454 @default.
• W1968206876 hasConcept C136009344 @default.
• W1968206876 hasConcept C142613039 @default.
• W1968206876 hasConcept C1491633281 @default.
• W1968206876 hasConcept C15526883 @default.
• W1968206876 hasConcept C169274487 @default.
• W1968206876 hasConcept C169760540 @default.
• W1968206876 hasConcept C196843134 @default.
• W1968206876 hasConcept C2524010 @default.
• W1968206876 hasConcept C2779178360 @default.
• W1968206876 hasConcept C2780594696 @default.
• W1968206876 hasConcept C2780775167 @default.
• W1968206876 hasConcept C30503417 @default.
• W1968206876 hasConcept C33923547 @default.
• W1968206876 hasConcept C47785574 @default.
• W1968206876 hasConcept C54355233 @default.
• W1968206876 hasConcept C55493867 @default.
• W1968206876 hasConcept C62520636 @default.
• W1968206876 hasConcept C86803240 @default.
• W1968206876 hasConcept C91881484 @default.
• W1968206876 hasConcept C95444343 @default.
• W1968206876 hasConceptScore W1968206876C103378370 @default.
• W1968206876 hasConceptScore W1968206876C104317684 @default.
• W1968206876 hasConceptScore W1968206876C105702510 @default.
• W1968206876 hasConceptScore W1968206876C113976765 @default.
• W1968206876 hasConceptScore W1968206876C121332964 @default.
• W1968206876 hasConceptScore W1968206876C126749454 @default.
• W1968206876 hasConceptScore W1968206876C136009344 @default.
• W1968206876 hasConceptScore W1968206876C142613039 @default.
• W1968206876 hasConceptScore W1968206876C1491633281 @default.
• W1968206876 hasConceptScore W1968206876C15526883 @default.
• W1968206876 hasConceptScore W1968206876C169274487 @default.

• next