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- W1968243212 abstract "A method for detecting glycosphing3lipids (GSL) in situ after thin layer chromatography is described. The separated GSL are transferred by diffusion to nitrocellulose. The replica is incubated with poly- or monoclonal antibodies and bound antibodies are detected with second antibodies coupled to peroxidase. Advantages of the procedure are its speed, the non-radioactive detection method, and its suitability for screening applications. In addition, small scale affinity purification of antibodies from the replicas is possible. The presence of Forssman antigen in mouse tissues and the reaction of monoclonal antibodies with human GSL is demonstrated." @default.
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- W1968243212 date "1984-09-01" @default.
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- W1968243212 title "Glycosphingolipid-blotting: an immunological detection procedure after separation by thin layer chromatography" @default.
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- W1968243212 doi "https://doi.org/10.1016/0022-1759(84)90015-2" @default.
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