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• W1968245655 abstract "Concanavalin A (Con A) has been used to rapidly and selectively agglutinate murine and avian oncornavirions from culture medium or plasma. The agglutinated virus was concentrated rapidly and gently by low-speed centrifugation and solubilization with α-methyl mannoside. Infectious virus was purified 2.3 times with respect to nucleic-acid content, and more than 60% of its infectivity was recovered. Infectious particles of densities 1.18 and 1.16 g/cm 3 were found in mouse cells infected with Friend virus. Con A reacted only with particles of density 1.16 g/cm 3 , indicating heterogeneity with respect to carbohydrate content or structure as well as buoyant density. Electron microscopy of virus agglutinated with Con A showed a zone of Con A-glycoprotein complexes averaging 12-15 nm in thickness." @default.
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• W1968245655 date "1973-05-01" @default.
• W1968245655 modified "2023-10-03" @default.
• W1968245655 title "Purification of Oncornaviruses by Agglutination with Concanavalin A" @default.
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• W1968245655 doi "https://doi.org/10.1073/pnas.70.5.1308" @default.
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