Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968258547> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1968258547 abstract "Argonaute (Ago) is the catalytic core of small RNA-based gene regulation. Despite a plenty of mechanistic studies on Ago, the dynamical aspects and the mechanistic determinants of target binding and dissociation of Ago-guide remain unclear. Here, by using single-molecule fluorescence resonance transfer (FRET) assays and Thermus thermophilus Ago, we reveal that Ago-guide dynamically associates and dissociates with a target, and that different regions of guide-target base pairing are responsible for different steps of target recognition by Ago-guide. In addition, we show that the 3’-end of the guide strand dynamically anchors at and releases from the PAZ domain of Ago, and that the 3’-end anchoring of the guide strand greatly accelerates the target dissociation by destabilizing the guide-target duplex. Collectively, our results implicate that the target binding/dissociation of Ago-guide is executed through the dynamic interplays among Ago, guide, and target." @default.
- W1968258547 created "2016-06-24" @default.
- W1968258547 creator A5001090654 @default.
- W1968258547 creator A5012934386 @default.
- W1968258547 creator A5014643514 @default.
- W1968258547 creator A5037358268 @default.
- W1968258547 creator A5058282307 @default.
- W1968258547 date "2013-01-01" @default.
- W1968258547 modified "2023-09-28" @default.
- W1968258547 title "Single Molecule Observation of Interaction between Argonaute-Guide and Target RNA and its Implication on Gene Silencing" @default.
- W1968258547 doi "https://doi.org/10.1016/j.bpj.2012.11.1117" @default.
- W1968258547 hasPublicationYear "2013" @default.
- W1968258547 type Work @default.
- W1968258547 sameAs 1968258547 @default.
- W1968258547 citedByCount "0" @default.
- W1968258547 crossrefType "journal-article" @default.
- W1968258547 hasAuthorship W1968258547A5001090654 @default.
- W1968258547 hasAuthorship W1968258547A5012934386 @default.
- W1968258547 hasAuthorship W1968258547A5014643514 @default.
- W1968258547 hasAuthorship W1968258547A5037358268 @default.
- W1968258547 hasAuthorship W1968258547A5058282307 @default.
- W1968258547 hasBestOaLocation W19682585471 @default.
- W1968258547 hasConcept C104317684 @default.
- W1968258547 hasConcept C119056186 @default.
- W1968258547 hasConcept C121332964 @default.
- W1968258547 hasConcept C12554922 @default.
- W1968258547 hasConcept C141231307 @default.
- W1968258547 hasConcept C144501496 @default.
- W1968258547 hasConcept C166703698 @default.
- W1968258547 hasConcept C201853390 @default.
- W1968258547 hasConcept C2780883830 @default.
- W1968258547 hasConcept C54355233 @default.
- W1968258547 hasConcept C547475151 @default.
- W1968258547 hasConcept C62520636 @default.
- W1968258547 hasConcept C67705224 @default.
- W1968258547 hasConcept C70721500 @default.
- W1968258547 hasConcept C86803240 @default.
- W1968258547 hasConcept C91881484 @default.
- W1968258547 hasConcept C95444343 @default.
- W1968258547 hasConcept C96305047 @default.
- W1968258547 hasConcept C97702854 @default.
- W1968258547 hasConceptScore W1968258547C104317684 @default.
- W1968258547 hasConceptScore W1968258547C119056186 @default.
- W1968258547 hasConceptScore W1968258547C121332964 @default.
- W1968258547 hasConceptScore W1968258547C12554922 @default.
- W1968258547 hasConceptScore W1968258547C141231307 @default.
- W1968258547 hasConceptScore W1968258547C144501496 @default.
- W1968258547 hasConceptScore W1968258547C166703698 @default.
- W1968258547 hasConceptScore W1968258547C201853390 @default.
- W1968258547 hasConceptScore W1968258547C2780883830 @default.
- W1968258547 hasConceptScore W1968258547C54355233 @default.
- W1968258547 hasConceptScore W1968258547C547475151 @default.
- W1968258547 hasConceptScore W1968258547C62520636 @default.
- W1968258547 hasConceptScore W1968258547C67705224 @default.
- W1968258547 hasConceptScore W1968258547C70721500 @default.
- W1968258547 hasConceptScore W1968258547C86803240 @default.
- W1968258547 hasConceptScore W1968258547C91881484 @default.
- W1968258547 hasConceptScore W1968258547C95444343 @default.
- W1968258547 hasConceptScore W1968258547C96305047 @default.
- W1968258547 hasConceptScore W1968258547C97702854 @default.
- W1968258547 hasLocation W19682585471 @default.
- W1968258547 hasOpenAccess W1968258547 @default.
- W1968258547 hasPrimaryLocation W19682585471 @default.
- W1968258547 hasRelatedWork W1984393110 @default.
- W1968258547 hasRelatedWork W2078185190 @default.
- W1968258547 hasRelatedWork W2084938583 @default.
- W1968258547 hasRelatedWork W2108858759 @default.
- W1968258547 hasRelatedWork W2161033655 @default.
- W1968258547 hasRelatedWork W2213309418 @default.
- W1968258547 hasRelatedWork W2794930816 @default.
- W1968258547 hasRelatedWork W2890730173 @default.
- W1968258547 hasRelatedWork W2921808963 @default.
- W1968258547 hasRelatedWork W3031273907 @default.
- W1968258547 hasRelatedWork W3213496913 @default.
- W1968258547 isParatext "false" @default.
- W1968258547 isRetracted "false" @default.
- W1968258547 magId "1968258547" @default.
- W1968258547 workType "article" @default.