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• W1968315087 abstract "Aims: The objective of this study was to develop a novel synthetic growth medium for increased conidium production of the entompathogenic fungus Metarhizium anisopliae. The medium should simulate natural nutritional conditions. Methods and Results: Macro‐ and micronutrients were selected in concentrations found in insects or from sources which are believed to be advantageous for virulence. Medium development was guided by a problem‐oriented genetic algorithm (GA) implemented with ‘mutation’ and ‘recombination’ operators specific for this optimization problem. Following five optimization steps and a total of 181 medium variations, 19 synthetic media led to increased conidium production as compared with the standard Sabouraud dextrose agar medium: increases in conidia yields of up to 120% and a 17‐fold higher conidium production per square centimetre of mycelium were achieved. Rapid germination of conidia, conidial C : N ratio, as well as spore‐bound Pr1 protease production were monitored as proposed quality control markers for a virulent inoculum. Conclusions: It was demonstrated that our problem‐oriented GA is a powerful tool for the design of chemically defined cultivation media with special/enhanced properties for anamorphic fungi. Significance and Impact of the Study: This is the first study, which demonstrates the advantage of the use of a GA for the rapid design of solid synthetic media for maximum production of virulent conidia." @default.
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• W1968315087 date "2008-08-01" @default.
• W1968315087 modified "2023-10-17" @default.
• W1968315087 title "Algorithm-based design of novel synthetic media for<i>Metarhizium anisopliae</i>simulating its nutritional conditions in the environment" @default.
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• W1968315087 doi "https://doi.org/10.1111/j.1365-2672.2008.03764.x" @default.
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