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- W1968342362 abstract "A rapid method for the isolation of hydroxylysylpyridinoline and lysylridinoline from bone by liquid chromatographic methods is described. Decalcified bone is hydrolysed in 7 M hydrochloric acid. After evaporation of the acid, the high molecular mass dark coloured degradation products are removed by adsorption on non-polar adsorbents. The pyridinolines are separated from the majority of the amino acids by adsorption on cellulose. Separation of HP and LP is performed either by cation-exchange chromatography or by reversed-phase ion-pari chromatography. The pyridinoline containing fractions are desalted by size-exclusion chromatography. The progress of the hydrolytic cleavage of collagen and the optimal parameters for purification and separation were examined. As a result the existing method allows the isolation of high amounts of pyridinolines with low amounts of adsorbents and chemical within a short time." @default.
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- W1968342362 date "1997-04-01" @default.
- W1968342362 modified "2023-09-27" @default.
- W1968342362 title "Rapid method for the isolation of hydroxylysylpyridinoline and lysylpyridinoline from concentrated bone hydrolysates by liquid chromatographic techniques" @default.
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- W1968342362 doi "https://doi.org/10.1016/s0378-4347(96)00456-2" @default.
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