Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968346052> ?p ?o ?g. }
- W1968346052 endingPage "9383" @default.
- W1968346052 startingPage "9377" @default.
- W1968346052 abstract "Proteins exist in a delicate balance between the native and unfolded states, where thermodynamic stability may be sacrificed to attain the flexibility required for efficient catalysis, binding, or allosteric control. Partition-defective 6 (Par-6) regulates the Par polarity complex by transmitting a GTPase signal through the Cdc42/Rac interaction binding PSD-95/Dlg/ZO-1 (CRIB-PDZ) module that alters PDZ ligand binding. Allosteric activation of the PDZ is achieved by local rearrangement of the L164 and K165 side chains to stabilize the interdomain CRIB:PDZ interface and reposition a conserved element of the ligand binding pocket. However, microsecond to millisecond dynamics measurements revealed that L164/K165 exchange requires a larger rearrangement than expected. The margin of thermodynamic stability for the PDZ domain is modest (∼3 kcal/mol) and further reduced by transient interactions with the disordered CRIB domain. Measurements of local structural stability revealed that tertiary contacts within the PDZ are disrupted by a partial unfolding transition that enables interconversion of the L/K switch. The unexpected participation of partial PDZ unfolding in the allosteric mechanism of Par-6 suggests that native-state unfolding may be essential for the function of other marginally stable proteins." @default.
- W1968346052 created "2016-06-24" @default.
- W1968346052 creator A5007750328 @default.
- W1968346052 creator A5042069730 @default.
- W1968346052 creator A5048853247 @default.
- W1968346052 creator A5088703972 @default.
- W1968346052 date "2013-06-12" @default.
- W1968346052 modified "2023-09-26" @default.
- W1968346052 title "Allosteric Activation of the Par-6 PDZ via a Partial Unfolding Transition" @default.
- W1968346052 cites W1549268831 @default.
- W1968346052 cites W1554521867 @default.
- W1968346052 cites W1577699365 @default.
- W1968346052 cites W1965410387 @default.
- W1968346052 cites W1970562061 @default.
- W1968346052 cites W1976470237 @default.
- W1968346052 cites W1977179882 @default.
- W1968346052 cites W1979511698 @default.
- W1968346052 cites W1979666917 @default.
- W1968346052 cites W1979757174 @default.
- W1968346052 cites W1991660106 @default.
- W1968346052 cites W1994939140 @default.
- W1968346052 cites W1997316839 @default.
- W1968346052 cites W2004373243 @default.
- W1968346052 cites W2008117836 @default.
- W1968346052 cites W2020464495 @default.
- W1968346052 cites W2035429746 @default.
- W1968346052 cites W2039200048 @default.
- W1968346052 cites W2044674664 @default.
- W1968346052 cites W2044713411 @default.
- W1968346052 cites W2053004995 @default.
- W1968346052 cites W2059601372 @default.
- W1968346052 cites W2068361985 @default.
- W1968346052 cites W2069384750 @default.
- W1968346052 cites W2075271795 @default.
- W1968346052 cites W2075797983 @default.
- W1968346052 cites W2081866413 @default.
- W1968346052 cites W2093047483 @default.
- W1968346052 cites W2093174095 @default.
- W1968346052 cites W2094655254 @default.
- W1968346052 cites W2097735426 @default.
- W1968346052 cites W2098731951 @default.
- W1968346052 cites W2098857316 @default.
- W1968346052 cites W2103313694 @default.
- W1968346052 cites W2105717859 @default.
- W1968346052 cites W2114320763 @default.
- W1968346052 cites W2121393047 @default.
- W1968346052 cites W2132186283 @default.
- W1968346052 cites W2139768945 @default.
- W1968346052 cites W2169821755 @default.
- W1968346052 cites W2171610577 @default.
- W1968346052 cites W30334872 @default.
- W1968346052 doi "https://doi.org/10.1021/ja400092a" @default.
- W1968346052 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3736553" @default.
- W1968346052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23705660" @default.
- W1968346052 hasPublicationYear "2013" @default.
- W1968346052 type Work @default.
- W1968346052 sameAs 1968346052 @default.
- W1968346052 citedByCount "9" @default.
- W1968346052 countsByYear W19683460522015 @default.
- W1968346052 countsByYear W19683460522016 @default.
- W1968346052 countsByYear W19683460522019 @default.
- W1968346052 countsByYear W19683460522021 @default.
- W1968346052 countsByYear W19683460522023 @default.
- W1968346052 crossrefType "journal-article" @default.
- W1968346052 hasAuthorship W1968346052A5007750328 @default.
- W1968346052 hasAuthorship W1968346052A5042069730 @default.
- W1968346052 hasAuthorship W1968346052A5048853247 @default.
- W1968346052 hasAuthorship W1968346052A5088703972 @default.
- W1968346052 hasBestOaLocation W19683460522 @default.
- W1968346052 hasConcept C116569031 @default.
- W1968346052 hasConcept C12554922 @default.
- W1968346052 hasConcept C154428179 @default.
- W1968346052 hasConcept C166342909 @default.
- W1968346052 hasConcept C170493617 @default.
- W1968346052 hasConcept C185592680 @default.
- W1968346052 hasConcept C207332259 @default.
- W1968346052 hasConcept C2780040266 @default.
- W1968346052 hasConcept C55493867 @default.
- W1968346052 hasConcept C71240020 @default.
- W1968346052 hasConcept C8010536 @default.
- W1968346052 hasConcept C86803240 @default.
- W1968346052 hasConceptScore W1968346052C116569031 @default.
- W1968346052 hasConceptScore W1968346052C12554922 @default.
- W1968346052 hasConceptScore W1968346052C154428179 @default.
- W1968346052 hasConceptScore W1968346052C166342909 @default.
- W1968346052 hasConceptScore W1968346052C170493617 @default.
- W1968346052 hasConceptScore W1968346052C185592680 @default.
- W1968346052 hasConceptScore W1968346052C207332259 @default.
- W1968346052 hasConceptScore W1968346052C2780040266 @default.
- W1968346052 hasConceptScore W1968346052C55493867 @default.
- W1968346052 hasConceptScore W1968346052C71240020 @default.
- W1968346052 hasConceptScore W1968346052C8010536 @default.
- W1968346052 hasConceptScore W1968346052C86803240 @default.
- W1968346052 hasIssue "25" @default.
- W1968346052 hasLocation W19683460521 @default.
- W1968346052 hasLocation W19683460522 @default.
- W1968346052 hasLocation W19683460523 @default.
- W1968346052 hasLocation W19683460524 @default.