Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968352752> ?p ?o ?g. }
- W1968352752 endingPage "426" @default.
- W1968352752 startingPage "418" @default.
- W1968352752 abstract "Insulin-like growth factors (IGFs) I and II (IGF-I and IGF-II) play important roles in fish growth and development. The present study was aimed at isolating cDNAs encoding both IGF-I and IGF-II in the redbanded seabream (Pagrus auriga), and at measuring relative gene expression levels in different organs and during larval development. A fragment of 1321 nucleotides coding for IGF-I was cloned from liver using 3′ and 5′ RACE techniques. It included an open reading frame of 558 nucleotides, encoding a 185-amino acid preproIGF-I. With respect to IGF-II, a fragment of 1544 nucleotides was cloned as well. The open reading frame spanned 648 nucleotides, rendering a 215-amino acid preproIGF-II. The deduced mature 67-amino acid IGF-I and 70-amino acid IGF-II exhibited high sequence identities with their corresponding fish counterparts, ranging between 88.6-100% and 79.1–98.5%, respectively. Real-time PCR showed the highest IGF-I transcripts in liver (∼ 200-fold higher than head-kidney). In contrast, the highest IGF-II mRNAs were detected in gills and heart (∼ 16-fold higher than head-kidney). In addition, both IGFs exhibited different gene expression patterns during larval development suggesting that their expression is developmentally regulated. IGF-I reached the highest expression levels at 18 days after hatching (11.6-fold higher than 1 day after hatching), whereas IGF-II expression did not change significantly. Both hepatic IGF-I and IGF-II mRNA levels increased sharply (3.1- and 19-fold higher than control, respectively) 3 h after injection of porcine growth hormone, but remained unchanged from 6 to 24 h after treatment. Our results are discussed in relation to those previously reported for other bony fish." @default.
- W1968352752 created "2016-06-24" @default.
- W1968352752 creator A5062765341 @default.
- W1968352752 creator A5064492628 @default.
- W1968352752 creator A5071873986 @default.
- W1968352752 creator A5091616832 @default.
- W1968352752 date "2008-08-01" @default.
- W1968352752 modified "2023-10-10" @default.
- W1968352752 title "Molecular characterization and gene expression analysis of insulin-like growth factors I and II in the redbanded seabream, Pagrus auriga: transcriptional regulation by growth hormone" @default.
- W1968352752 cites W1509707197 @default.
- W1968352752 cites W1563869447 @default.
- W1968352752 cites W1966108514 @default.
- W1968352752 cites W1966351724 @default.
- W1968352752 cites W1969036078 @default.
- W1968352752 cites W1978401355 @default.
- W1968352752 cites W1978787303 @default.
- W1968352752 cites W1978824786 @default.
- W1968352752 cites W1979687455 @default.
- W1968352752 cites W1983448400 @default.
- W1968352752 cites W1987889221 @default.
- W1968352752 cites W1994606300 @default.
- W1968352752 cites W1996831533 @default.
- W1968352752 cites W1998236500 @default.
- W1968352752 cites W1999846688 @default.
- W1968352752 cites W2001049413 @default.
- W1968352752 cites W2001454442 @default.
- W1968352752 cites W2003364505 @default.
- W1968352752 cites W2006026931 @default.
- W1968352752 cites W2006447700 @default.
- W1968352752 cites W2007573212 @default.
- W1968352752 cites W2012628315 @default.
- W1968352752 cites W2016616818 @default.
- W1968352752 cites W2016867317 @default.
- W1968352752 cites W2018191058 @default.
- W1968352752 cites W2020298493 @default.
- W1968352752 cites W2023286170 @default.
- W1968352752 cites W2024542975 @default.
- W1968352752 cites W2024956365 @default.
- W1968352752 cites W2028728132 @default.
- W1968352752 cites W2036523102 @default.
- W1968352752 cites W2040607511 @default.
- W1968352752 cites W2047561346 @default.
- W1968352752 cites W2055606852 @default.
- W1968352752 cites W2061031951 @default.
- W1968352752 cites W2063719992 @default.
- W1968352752 cites W2065537773 @default.
- W1968352752 cites W2066383609 @default.
- W1968352752 cites W2070905333 @default.
- W1968352752 cites W2072240356 @default.
- W1968352752 cites W2072716857 @default.
- W1968352752 cites W2073247886 @default.
- W1968352752 cites W2076957285 @default.
- W1968352752 cites W2078368871 @default.
- W1968352752 cites W2078602273 @default.
- W1968352752 cites W2078893959 @default.
- W1968352752 cites W2084516660 @default.
- W1968352752 cites W2084962606 @default.
- W1968352752 cites W2085209617 @default.
- W1968352752 cites W2085975829 @default.
- W1968352752 cites W2092259056 @default.
- W1968352752 cites W2094397243 @default.
- W1968352752 cites W2095935167 @default.
- W1968352752 cites W2098586878 @default.
- W1968352752 cites W2103557938 @default.
- W1968352752 cites W2104998259 @default.
- W1968352752 cites W2106920798 @default.
- W1968352752 cites W2107277218 @default.
- W1968352752 cites W2109106935 @default.
- W1968352752 cites W2118384294 @default.
- W1968352752 cites W2121484931 @default.
- W1968352752 cites W2124824608 @default.
- W1968352752 cites W2133292623 @default.
- W1968352752 cites W2137649869 @default.
- W1968352752 cites W2137763501 @default.
- W1968352752 cites W2138338566 @default.
- W1968352752 cites W2145773598 @default.
- W1968352752 cites W2158252316 @default.
- W1968352752 cites W2163035954 @default.
- W1968352752 cites W2164507972 @default.
- W1968352752 cites W218014608 @default.
- W1968352752 cites W278729645 @default.
- W1968352752 cites W278758063 @default.
- W1968352752 doi "https://doi.org/10.1016/j.cbpb.2008.04.013" @default.
- W1968352752 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18539063" @default.
- W1968352752 hasPublicationYear "2008" @default.
- W1968352752 type Work @default.
- W1968352752 sameAs 1968352752 @default.
- W1968352752 citedByCount "30" @default.
- W1968352752 countsByYear W19683527522012 @default.
- W1968352752 countsByYear W19683527522013 @default.
- W1968352752 countsByYear W19683527522014 @default.
- W1968352752 countsByYear W19683527522016 @default.
- W1968352752 countsByYear W19683527522017 @default.
- W1968352752 countsByYear W19683527522018 @default.
- W1968352752 countsByYear W19683527522021 @default.
- W1968352752 crossrefType "journal-article" @default.
- W1968352752 hasAuthorship W1968352752A5062765341 @default.
- W1968352752 hasAuthorship W1968352752A5064492628 @default.