FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968355797> ?p ?o ?g. }

• W1968355797 endingPage "105" @default.
• W1968355797 startingPage "95" @default.
• W1968355797 abstract "The SLC30A8 gene encodes the zinc transporter ZnT-8, which provides zinc for insulin-hexamer formation. Genome-wide association studies have shown that a polymorphic variant in SLC30A8 is associated with altered susceptibility to Type 2 diabetes and we recently reported that glucose-stimulated insulin secretion is decreased in islets isolated from Slc30a8-knockout mice. The present study examines the molecular basis for the islet-specific expression of Slc30a8. VISTA analyses identified two conserved regions in Slc30a8 introns 2 and 3, designated enhancers A and B respectively. Transfection experiments demonstrated that enhancer B confers elevated fusion gene expression in both βTC-3 cells and αTC-6 cells. In contrast, enhancer A confers elevated fusion gene expression selectively in βTC-3 and not αTC-6 cells. These data suggest that enhancer A is an islet β-cell-specific enhancer and that the mechanisms controlling Slc30a8 expression in α- and β-cells are overlapping, but distinct. Gel retardation and ChIP (chromatin immunoprecipitation) assays revealed that the islet-enriched transcription factor Pdx-1 binds enhancer A in vitro and in situ respectively. Mutation of two Pdx-1-binding sites in enhancer A markedly reduces fusion gene expression suggesting that this factor contributes to Slc30a8 expression in β-cells, a conclusion consistent with developmental studies showing that restriction of Pdx-1 to pancreatic islet β-cells correlates with the induction of Slc30a8 gene expression and ZnT-8 protein expression in vivo." @default.
• W1968355797 created "2016-06-24" @default.
• W1968355797 creator A5018190018 @default.
• W1968355797 creator A5031203340 @default.
• W1968355797 creator A5032143500 @default.
• W1968355797 creator A5039680631 @default.
• W1968355797 creator A5041458515 @default.
• W1968355797 creator A5044915862 @default.
• W1968355797 creator A5061340857 @default.
• W1968355797 creator A5062511690 @default.
• W1968355797 creator A5069819556 @default.
• W1968355797 creator A5077843010 @default.
• W1968355797 creator A5079693358 @default.
• W1968355797 date "2010-12-15" @default.
• W1968355797 modified "2023-09-26" @default.
• W1968355797 title "The pancreatic islet β-cell-enriched transcription factor Pdx-1 regulates <i>Slc30a8</i> gene transcription through an intronic enhancer" @default.
• W1968355797 cites W1565835249 @default.
• W1968355797 cites W1573536022 @default.
• W1968355797 cites W1944558458 @default.
• W1968355797 cites W1961434948 @default.
• W1968355797 cites W1969770714 @default.
• W1968355797 cites W1971465107 @default.
• W1968355797 cites W1978393827 @default.
• W1968355797 cites W1983092821 @default.
• W1968355797 cites W1989257844 @default.
• W1968355797 cites W1992517228 @default.
• W1968355797 cites W2003207229 @default.
• W1968355797 cites W2005227623 @default.
• W1968355797 cites W2012154903 @default.
• W1968355797 cites W2014182850 @default.
• W1968355797 cites W2019028906 @default.
• W1968355797 cites W2020542551 @default.
• W1968355797 cites W2022633562 @default.
• W1968355797 cites W2025143098 @default.
• W1968355797 cites W2027448315 @default.
• W1968355797 cites W2031204411 @default.
• W1968355797 cites W2038322995 @default.
• W1968355797 cites W2038862314 @default.
• W1968355797 cites W2042009748 @default.
• W1968355797 cites W2048755332 @default.
• W1968355797 cites W2066752848 @default.
• W1968355797 cites W2082122713 @default.
• W1968355797 cites W2083490046 @default.
• W1968355797 cites W2090350940 @default.
• W1968355797 cites W2092435609 @default.
• W1968355797 cites W2094943786 @default.
• W1968355797 cites W2103306919 @default.
• W1968355797 cites W2103600331 @default.
• W1968355797 cites W2106073742 @default.
• W1968355797 cites W2109679032 @default.
• W1968355797 cites W2119503252 @default.
• W1968355797 cites W2119993037 @default.
• W1968355797 cites W2130595746 @default.
• W1968355797 cites W2134722403 @default.
• W1968355797 cites W2134857194 @default.
• W1968355797 cites W2137761662 @default.
• W1968355797 cites W2139943786 @default.
• W1968355797 cites W2144576542 @default.
• W1968355797 cites W2144594789 @default.
• W1968355797 cites W2144958072 @default.
• W1968355797 cites W2146127531 @default.
• W1968355797 cites W2149006664 @default.
• W1968355797 cites W2158982987 @default.
• W1968355797 cites W2166421062 @default.
• W1968355797 cites W2466174852 @default.
• W1968355797 doi "https://doi.org/10.1042/bj20101488" @default.
• W1968355797 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4130494" @default.
• W1968355797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20942803" @default.
• W1968355797 hasPublicationYear "2010" @default.
• W1968355797 type Work @default.
• W1968355797 sameAs 1968355797 @default.
• W1968355797 citedByCount "29" @default.
• W1968355797 countsByYear W19683557972012 @default.
• W1968355797 countsByYear W19683557972013 @default.
• W1968355797 countsByYear W19683557972014 @default.
• W1968355797 countsByYear W19683557972015 @default.
• W1968355797 countsByYear W19683557972016 @default.
• W1968355797 countsByYear W19683557972017 @default.
• W1968355797 countsByYear W19683557972019 @default.
• W1968355797 countsByYear W19683557972020 @default.
• W1968355797 countsByYear W19683557972021 @default.
• W1968355797 countsByYear W19683557972022 @default.
• W1968355797 countsByYear W19683557972023 @default.
• W1968355797 crossrefType "journal-article" @default.
• W1968355797 hasAuthorship W1968355797A5018190018 @default.
• W1968355797 hasAuthorship W1968355797A5031203340 @default.
• W1968355797 hasAuthorship W1968355797A5032143500 @default.
• W1968355797 hasAuthorship W1968355797A5039680631 @default.
• W1968355797 hasAuthorship W1968355797A5041458515 @default.
• W1968355797 hasAuthorship W1968355797A5044915862 @default.
• W1968355797 hasAuthorship W1968355797A5061340857 @default.
• W1968355797 hasAuthorship W1968355797A5062511690 @default.
• W1968355797 hasAuthorship W1968355797A5069819556 @default.
• W1968355797 hasAuthorship W1968355797A5077843010 @default.
• W1968355797 hasAuthorship W1968355797A5079693358 @default.
• W1968355797 hasBestOaLocation W19683557972 @default.
• W1968355797 hasConcept C104317684 @default.
• W1968355797 hasConcept C111936080 @default.

• next