FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968360052> ?p ?o ?g. }

• W1968360052 endingPage "169" @default.
• W1968360052 startingPage "162" @default.
• W1968360052 abstract "Phospholipase D (PLD) is one of the main enzymes involved in signal transduction, vesicle trafficking and membrane metabolism processes. Here we describe the heterologous high-yield expression in the yeast Pichia pastoris, one-step purification and characterization of catalytically active PLDα from cowpea (Vigna unguiculata L. Walp). Immunoblotting experiments showed that recombinant PLDα is recognized by a polyclonal antibody raised against native soybean PLDα. A single calcium-dependent octyl-Sepharose chromatography step was used to obtain a highly purified recombinant PLDα, as attested by gel electrophoresis, N-terminal amino acid sequence and mass spectrometry data. From 1 L of yeast culture medium, about 8 mg of pure recombinant PLDα was obtained and the specific activity measured on phosphatidylcholine was 27 μmol/min/mg. Contrary to what was observed previously with Vigna unguiculata PLDα expressed in insect cells, no proteolytic degradation of the N-terminal calcium-dependent C2 lipid binding domain was observed here. This functional recombinant PLDα should provide a valuable tool for performing detailed studies on the molecular characterization of enzymes as well as structural studies." @default.
• W1968360052 created "2016-06-24" @default.
• W1968360052 creator A5002034222 @default.
• W1968360052 creator A5014211286 @default.
• W1968360052 creator A5055085542 @default.
• W1968360052 date "2007-02-01" @default.
• W1968360052 modified "2023-10-17" @default.
• W1968360052 title "High-level constitutive expression in Pichia pastoris and one-step purification of phospholipase D from cowpea (Vigna unguiculata L. Walp)" @default.
• W1968360052 cites W1495874496 @default.
• W1968360052 cites W1503820853 @default.
• W1968360052 cites W1695689747 @default.
• W1968360052 cites W1967703956 @default.
• W1968360052 cites W1971182645 @default.
• W1968360052 cites W1991703472 @default.
• W1968360052 cites W1995610541 @default.
• W1968360052 cites W2005593816 @default.
• W1968360052 cites W2012840382 @default.
• W1968360052 cites W2017543679 @default.
• W1968360052 cites W2017749309 @default.
• W1968360052 cites W2019420003 @default.
• W1968360052 cites W2045532688 @default.
• W1968360052 cites W2069529565 @default.
• W1968360052 cites W2070603326 @default.
• W1968360052 cites W2077225959 @default.
• W1968360052 cites W2080692650 @default.
• W1968360052 cites W2092047144 @default.
• W1968360052 cites W2100837269 @default.
• W1968360052 cites W2105460434 @default.
• W1968360052 cites W2141721390 @default.
• W1968360052 cites W2168739774 @default.
• W1968360052 cites W2198827822 @default.
• W1968360052 cites W2328063168 @default.
• W1968360052 cites W278464241 @default.
• W1968360052 cites W306292651 @default.
• W1968360052 cites W4293247451 @default.
• W1968360052 cites W94305437 @default.
• W1968360052 doi "https://doi.org/10.1016/j.pep.2006.07.018" @default.
• W1968360052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16949301" @default.
• W1968360052 hasPublicationYear "2007" @default.
• W1968360052 type Work @default.
• W1968360052 sameAs 1968360052 @default.
• W1968360052 citedByCount "13" @default.
• W1968360052 countsByYear W19683600522012 @default.
• W1968360052 countsByYear W19683600522015 @default.
• W1968360052 countsByYear W19683600522016 @default.
• W1968360052 countsByYear W19683600522018 @default.
• W1968360052 countsByYear W19683600522019 @default.
• W1968360052 countsByYear W19683600522021 @default.
• W1968360052 crossrefType "journal-article" @default.
• W1968360052 hasAuthorship W1968360052A5002034222 @default.
• W1968360052 hasAuthorship W1968360052A5014211286 @default.
• W1968360052 hasAuthorship W1968360052A5055085542 @default.
• W1968360052 hasConcept C104317684 @default.
• W1968360052 hasConcept C108305142 @default.
• W1968360052 hasConcept C153911025 @default.
• W1968360052 hasConcept C159654299 @default.
• W1968360052 hasConcept C181199279 @default.
• W1968360052 hasConcept C203014093 @default.
• W1968360052 hasConcept C2779222958 @default.
• W1968360052 hasConcept C2779703480 @default.
• W1968360052 hasConcept C2779862049 @default.
• W1968360052 hasConcept C2781372317 @default.
• W1968360052 hasConcept C40767141 @default.
• W1968360052 hasConcept C55493867 @default.
• W1968360052 hasConcept C6182249 @default.
• W1968360052 hasConcept C6557445 @default.
• W1968360052 hasConcept C86803240 @default.
• W1968360052 hasConcept C956191 @default.
• W1968360052 hasConceptScore W1968360052C104317684 @default.
• W1968360052 hasConceptScore W1968360052C108305142 @default.
• W1968360052 hasConceptScore W1968360052C153911025 @default.
• W1968360052 hasConceptScore W1968360052C159654299 @default.
• W1968360052 hasConceptScore W1968360052C181199279 @default.
• W1968360052 hasConceptScore W1968360052C203014093 @default.
• W1968360052 hasConceptScore W1968360052C2779222958 @default.
• W1968360052 hasConceptScore W1968360052C2779703480 @default.
• W1968360052 hasConceptScore W1968360052C2779862049 @default.
• W1968360052 hasConceptScore W1968360052C2781372317 @default.
• W1968360052 hasConceptScore W1968360052C40767141 @default.
• W1968360052 hasConceptScore W1968360052C55493867 @default.
• W1968360052 hasConceptScore W1968360052C6182249 @default.
• W1968360052 hasConceptScore W1968360052C6557445 @default.
• W1968360052 hasConceptScore W1968360052C86803240 @default.
• W1968360052 hasConceptScore W1968360052C956191 @default.
• W1968360052 hasIssue "2" @default.
• W1968360052 hasLocation W19683600521 @default.
• W1968360052 hasLocation W19683600522 @default.
• W1968360052 hasOpenAccess W1968360052 @default.
• W1968360052 hasPrimaryLocation W19683600521 @default.
• W1968360052 hasRelatedWork W1480951157 @default.
• W1968360052 hasRelatedWork W1975687798 @default.
• W1968360052 hasRelatedWork W2044140285 @default.
• W1968360052 hasRelatedWork W2256808269 @default.
• W1968360052 hasRelatedWork W2363862718 @default.
• W1968360052 hasRelatedWork W2368039950 @default.
• W1968360052 hasRelatedWork W2369041049 @default.
• W1968360052 hasRelatedWork W2393867567 @default.
• W1968360052 hasRelatedWork W2394330041 @default.

• next