FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968360788> ?p ?o ?g. }

• W1968360788 endingPage "824" @default.
• W1968360788 startingPage "813" @default.
• W1968360788 abstract "The objective of the present study was to assess the in vitro viability of ovine embryos at different stages of development after combining cell sampling and vitrification. Precompacted morulae, compacted morulae and blastocysts were obtained from superovulated Sarda ewes at 4, 5 or 6 d following insemination. Embryo cell biopsy was carried out in a 100-μl drop of PBS + 10% fetal calf serum (FCS) with 10 μmol nocodazole and 7.5 μg/ml cytochalasin-b by aspiration (3–5 cells). Embryos were cryopreserved at room temperature after exposure of 2 solutions for 5 min, transferred into a vitrification solution, loaded into the center of 0.25-ml straws separated by air bubbles from 2 columns of sucrose 0.5 M and plunged immediately into liquid nitrogen. In Experiment 1, the in vitro viability of manipulated or vitrified embryos after in vitro co-culture in TCM 199 medium with 10% FCS and sheep oviductal epithelial cells (SOEC) in 5% CO2 humidified atmosphere in air at 39 °C was significantly lower (P < 0.05 and P < 0.01, respectively) at precompacted morula (60 and 30%) and compacted morula (62 and 39%) stages than intact embryos at the same stages (87 and 88%). No differences were found at the blastocyst stage. In Experiment 2, the in vitro survival rate of precompacted morulae which were manipulated and immediately vitrified was lower (P < 0.05) than in those manipulated and, after a temporary period of culture, vitrified at blastocyst stage (21 vs 48%); while no differences were found at compacted morula and blastocyst stages. The results show that 1) the stage of development influences the subsequent in vitro viability of manipulated and vitrified ovine embryos, 2) temporary culture after manipulation and before vitrification improves the in vitro viability of embryos, and 3) the hole in the zona pellucida resulting from biopsy does not affect blastocyst survival after subsequent vitrification." @default.
• W1968360788 created "2016-06-24" @default.
• W1968360788 creator A5033801455 @default.
• W1968360788 creator A5046654698 @default.
• W1968360788 creator A5056100459 @default.
• W1968360788 creator A5061082633 @default.
• W1968360788 creator A5062924194 @default.
• W1968360788 creator A5077634975 @default.
• W1968360788 creator A5090047612 @default.
• W1968360788 date "1996-10-01" @default.
• W1968360788 modified "2023-10-01" @default.
• W1968360788 title "Effect of biopsy and vitrification on in vitro survival of ovine embryos at different stages of development" @default.
• W1968360788 cites W1826989328 @default.
• W1968360788 cites W1859213739 @default.
• W1968360788 cites W1899795706 @default.
• W1968360788 cites W1965597021 @default.
• W1968360788 cites W1966677315 @default.
• W1968360788 cites W1980031981 @default.
• W1968360788 cites W1980053727 @default.
• W1968360788 cites W1981264510 @default.
• W1968360788 cites W1985071632 @default.
• W1968360788 cites W1999646916 @default.
• W1968360788 cites W2000215749 @default.
• W1968360788 cites W2006225405 @default.
• W1968360788 cites W2009057295 @default.
• W1968360788 cites W2018723904 @default.
• W1968360788 cites W2039013246 @default.
• W1968360788 cites W2039263318 @default.
• W1968360788 cites W2040855835 @default.
• W1968360788 cites W2046210812 @default.
• W1968360788 cites W2048332595 @default.
• W1968360788 cites W2050835280 @default.
• W1968360788 cites W2069477212 @default.
• W1968360788 cites W2069765302 @default.
• W1968360788 cites W2084544511 @default.
• W1968360788 cites W2091583005 @default.
• W1968360788 cites W2100237485 @default.
• W1968360788 cites W2106925907 @default.
• W1968360788 cites W2111451234 @default.
• W1968360788 cites W2126837259 @default.
• W1968360788 cites W2146170643 @default.
• W1968360788 doi "https://doi.org/10.1016/s0093-691x(96)00239-7" @default.
• W1968360788 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16727945" @default.
• W1968360788 hasPublicationYear "1996" @default.
• W1968360788 type Work @default.
• W1968360788 sameAs 1968360788 @default.
• W1968360788 citedByCount "25" @default.
• W1968360788 countsByYear W19683607882012 @default.
• W1968360788 countsByYear W19683607882013 @default.
• W1968360788 countsByYear W19683607882015 @default.
• W1968360788 countsByYear W19683607882017 @default.
• W1968360788 countsByYear W19683607882018 @default.
• W1968360788 countsByYear W19683607882019 @default.
• W1968360788 countsByYear W19683607882022 @default.
• W1968360788 countsByYear W19683607882023 @default.
• W1968360788 crossrefType "journal-article" @default.
• W1968360788 hasAuthorship W1968360788A5033801455 @default.
• W1968360788 hasAuthorship W1968360788A5046654698 @default.
• W1968360788 hasAuthorship W1968360788A5056100459 @default.
• W1968360788 hasAuthorship W1968360788A5061082633 @default.
• W1968360788 hasAuthorship W1968360788A5062924194 @default.
• W1968360788 hasAuthorship W1968360788A5077634975 @default.
• W1968360788 hasAuthorship W1968360788A5090047612 @default.
• W1968360788 hasConcept C16685009 @default.
• W1968360788 hasConcept C179933525 @default.
• W1968360788 hasConcept C185592680 @default.
• W1968360788 hasConcept C196843134 @default.
• W1968360788 hasConcept C202751555 @default.
• W1968360788 hasConcept C2777005246 @default.
• W1968360788 hasConcept C2778022349 @default.
• W1968360788 hasConcept C2778177303 @default.
• W1968360788 hasConcept C2779012151 @default.
• W1968360788 hasConcept C2781087480 @default.
• W1968360788 hasConcept C55493867 @default.
• W1968360788 hasConcept C59843425 @default.
• W1968360788 hasConcept C71924100 @default.
• W1968360788 hasConcept C86803240 @default.
• W1968360788 hasConcept C87073359 @default.
• W1968360788 hasConcept C95444343 @default.
• W1968360788 hasConceptScore W1968360788C16685009 @default.
• W1968360788 hasConceptScore W1968360788C179933525 @default.
• W1968360788 hasConceptScore W1968360788C185592680 @default.
• W1968360788 hasConceptScore W1968360788C196843134 @default.
• W1968360788 hasConceptScore W1968360788C202751555 @default.
• W1968360788 hasConceptScore W1968360788C2777005246 @default.
• W1968360788 hasConceptScore W1968360788C2778022349 @default.
• W1968360788 hasConceptScore W1968360788C2778177303 @default.
• W1968360788 hasConceptScore W1968360788C2779012151 @default.
• W1968360788 hasConceptScore W1968360788C2781087480 @default.
• W1968360788 hasConceptScore W1968360788C55493867 @default.
• W1968360788 hasConceptScore W1968360788C59843425 @default.
• W1968360788 hasConceptScore W1968360788C71924100 @default.
• W1968360788 hasConceptScore W1968360788C86803240 @default.
• W1968360788 hasConceptScore W1968360788C87073359 @default.
• W1968360788 hasConceptScore W1968360788C95444343 @default.
• W1968360788 hasIssue "5" @default.
• W1968360788 hasLocation W19683607881 @default.
• W1968360788 hasLocation W19683607882 @default.

• next