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• W1968384490 abstract "ABSTRACT Our research on the mechanisms of action of chlorine-based oxidants on Cryptosporidium parvum oocysts in water revealed a dual-phase effect: (i) response to oxidative stress, which was demonstrated by induced expression of the Hsp70 heat shock gene, and (ii) oocyst inactivation as a result of long-term exposure to oxidants. The relative biocidal effects of sodium hypochlorite (bleach) and electrolytically generated mixed oxidant solution (MOS) on C. parvum oocysts were compared at identical free chlorine concentrations. Oocyst inactivation was determined by quantitative reverse transcription-PCR (qRT-PCR) amplification of the heat-induced Hsp70 mRNA and compared with tissue culture infectivity. According to both assays, within the range between 25 and 250 mg/liter free chlorine and with 4 h contact time, MOS exhibits a higher efficacy in oocyst inactivation than hypochlorite. Other RNA-based viability assays, aimed at monitoring the levels of β-tubulin mRNA and 18S rRNA, showed relatively slow decay rates of these molecules following disinfection by chlorine-based oxidants, rendering these molecular diagnostic viability markers inappropriate for disinfection efficacy assessment." @default.
• W1968384490 created "2016-06-24" @default.
• W1968384490 creator A5013287364 @default.
• W1968384490 creator A5043957034 @default.
• W1968384490 date "2010-03-15" @default.
• W1968384490 modified "2023-10-16" @default.
• W1968384490 title "Stress-Induced Hsp70 Gene Expression and Inactivation of <i>Cryptosporidium parvum</i> Oocysts by Chlorine-Based Oxidants" @default.
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• W1968384490 doi "https://doi.org/10.1128/aem.02353-09" @default.
• W1968384490 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2838021" @default.
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