Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968442921> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1968442921 endingPage "703" @default.
- W1968442921 startingPage "697" @default.
- W1968442921 abstract "Accumulation of cells containing metaphase chromosomes is an important step in cytological analyses and chromosome sorting procedures. The goal of this research was to optimize treatment parameters to synchronize the cell cycle of maize root tip meristem cells. Levels of hydroxyurea, a DNA synthesis inhibitor, were assessed for their utility in accumulating cells at the G1 phase of the cell cycle. Trifluralin, amiprophos-methyl, and colchicine were used to accumulate cells containing metaphase chromosomes upon release from hydroxyurea inhibition. Optimal mitotic indices were achieved by treating seedlings with 5 mM hydroxyurea for 18 h, incubating for 1 h without chemical treatment to release the hydroxyurea block, and then treating emerging roots with 1 μM trifluralin for 4 h. The mitotic index of synchronized maize root tips was over 70%. Uniformity of synchronization depended upon selection of seeds with emerging radicles that were similar in length at the time of treatment. Suspensions of intact chromosomes were prepared by a simple slicing procedure. The chromosome preparations were found to be suitable for flow cytometric characterization and sorting. Chromosome peaks of the observed flow karyotype resembled the predicted flow karyotype calculated on the basis of maize chromosome size. Key words : flow karyotype, hydroxyurea, plant chromosome sorting, trifluralin." @default.
- W1968442921 created "2016-06-24" @default.
- W1968442921 creator A5036071829 @default.
- W1968442921 creator A5050061476 @default.
- W1968442921 creator A5053857375 @default.
- W1968442921 creator A5085218579 @default.
- W1968442921 creator A5089160533 @default.
- W1968442921 date "1996-08-01" @default.
- W1968442921 modified "2023-09-24" @default.
- W1968442921 title "Cell synchronization and isolation of metaphase chromosomes from maize (<i>Zea mays</i> L.) root tips for flow cytometric analysis and sorting" @default.
- W1968442921 cites W1965161302 @default.
- W1968442921 cites W1973253466 @default.
- W1968442921 cites W1992763602 @default.
- W1968442921 cites W1999016710 @default.
- W1968442921 cites W2032153408 @default.
- W1968442921 cites W2032850402 @default.
- W1968442921 cites W2043894927 @default.
- W1968442921 cites W2045860229 @default.
- W1968442921 cites W2053144295 @default.
- W1968442921 cites W2078785924 @default.
- W1968442921 cites W2090832876 @default.
- W1968442921 cites W2116525133 @default.
- W1968442921 cites W2125694354 @default.
- W1968442921 doi "https://doi.org/10.1139/g96-088" @default.
- W1968442921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18469929" @default.
- W1968442921 hasPublicationYear "1996" @default.
- W1968442921 type Work @default.
- W1968442921 sameAs 1968442921 @default.
- W1968442921 citedByCount "51" @default.
- W1968442921 countsByYear W19684429212012 @default.
- W1968442921 countsByYear W19684429212014 @default.
- W1968442921 countsByYear W19684429212015 @default.
- W1968442921 countsByYear W19684429212016 @default.
- W1968442921 countsByYear W19684429212018 @default.
- W1968442921 countsByYear W19684429212019 @default.
- W1968442921 countsByYear W19684429212021 @default.
- W1968442921 countsByYear W19684429212022 @default.
- W1968442921 countsByYear W19684429212023 @default.
- W1968442921 crossrefType "journal-article" @default.
- W1968442921 hasAuthorship W1968442921A5036071829 @default.
- W1968442921 hasAuthorship W1968442921A5050061476 @default.
- W1968442921 hasAuthorship W1968442921A5053857375 @default.
- W1968442921 hasAuthorship W1968442921A5085218579 @default.
- W1968442921 hasAuthorship W1968442921A5089160533 @default.
- W1968442921 hasConcept C104317684 @default.
- W1968442921 hasConcept C147273371 @default.
- W1968442921 hasConcept C1491633281 @default.
- W1968442921 hasConcept C153911025 @default.
- W1968442921 hasConcept C2776425178 @default.
- W1968442921 hasConcept C29537977 @default.
- W1968442921 hasConcept C30481170 @default.
- W1968442921 hasConcept C53226629 @default.
- W1968442921 hasConcept C54355233 @default.
- W1968442921 hasConcept C6557445 @default.
- W1968442921 hasConcept C66640590 @default.
- W1968442921 hasConcept C70278784 @default.
- W1968442921 hasConcept C86803240 @default.
- W1968442921 hasConcept C88254577 @default.
- W1968442921 hasConcept C93304396 @default.
- W1968442921 hasConceptScore W1968442921C104317684 @default.
- W1968442921 hasConceptScore W1968442921C147273371 @default.
- W1968442921 hasConceptScore W1968442921C1491633281 @default.
- W1968442921 hasConceptScore W1968442921C153911025 @default.
- W1968442921 hasConceptScore W1968442921C2776425178 @default.
- W1968442921 hasConceptScore W1968442921C29537977 @default.
- W1968442921 hasConceptScore W1968442921C30481170 @default.
- W1968442921 hasConceptScore W1968442921C53226629 @default.
- W1968442921 hasConceptScore W1968442921C54355233 @default.
- W1968442921 hasConceptScore W1968442921C6557445 @default.
- W1968442921 hasConceptScore W1968442921C66640590 @default.
- W1968442921 hasConceptScore W1968442921C70278784 @default.
- W1968442921 hasConceptScore W1968442921C86803240 @default.
- W1968442921 hasConceptScore W1968442921C88254577 @default.
- W1968442921 hasConceptScore W1968442921C93304396 @default.
- W1968442921 hasIssue "4" @default.
- W1968442921 hasLocation W19684429211 @default.
- W1968442921 hasLocation W19684429212 @default.
- W1968442921 hasOpenAccess W1968442921 @default.
- W1968442921 hasPrimaryLocation W19684429211 @default.
- W1968442921 hasRelatedWork W1535879769 @default.
- W1968442921 hasRelatedWork W2020210364 @default.
- W1968442921 hasRelatedWork W2021348150 @default.
- W1968442921 hasRelatedWork W2287684967 @default.
- W1968442921 hasRelatedWork W2365200936 @default.
- W1968442921 hasRelatedWork W2380215229 @default.
- W1968442921 hasRelatedWork W2391336347 @default.
- W1968442921 hasRelatedWork W2393036521 @default.
- W1968442921 hasRelatedWork W2405161869 @default.
- W1968442921 hasRelatedWork W2411957037 @default.
- W1968442921 hasVolume "39" @default.
- W1968442921 isParatext "false" @default.
- W1968442921 isRetracted "false" @default.
- W1968442921 magId "1968442921" @default.
- W1968442921 workType "article" @default.