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- W1968531028 abstract "For thermostable lipase production by Humicola lanuginosa No. 3, a simple optimized medium consisting of (%, w/v): sorbitol, 1.0; corn steep liquor, 1.0; NaCl, 0.5; CaCl2•2H2O, 0.01; Silicone Km-70 (antifoamer), 0.2; and whale oil or castor oil as a lipase inducer, 0.3, was used. The yield of the lipase was about 80-120U/ml after 25 hr aerobic cultivation at 45°C when the pH was maintained at 7 to 8. The acetone powder preparation of the enzyme was most active at pH 7.0 and 45°C. The enzyme retained 100% activity on incubation for 20 hr at 60°C. The enzyme was able to hydrolyze almost all forms of natural fats tested (14 kinds), coconut oil being the most rapidly hydrolyzed." @default.
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- W1968531028 date "1987-01-01" @default.
- W1968531028 modified "2023-10-16" @default.
- W1968531028 title "Production of a thermostable lipase by Humicola lanuginosa grown on sorbitol-corn steep liquor medium." @default.
- W1968531028 cites W1566830427 @default.
- W1968531028 doi "https://doi.org/10.1271/bbb1961.51.2145" @default.
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