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• W1968532486 abstract "Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor." @default.
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• W1968532486 date "2013-05-01" @default.
• W1968532486 modified "2023-10-16" @default.
• W1968532486 title "Polyethylene imine-based receptor immobilization for label free bioassays" @default.
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• W1968532486 doi "https://doi.org/10.1016/j.snb.2012.12.097" @default.
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