Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968549005> ?p ?o ?g. }
- W1968549005 endingPage "784" @default.
- W1968549005 startingPage "773" @default.
- W1968549005 abstract "A key focus of research on pancreatic ductal adenocarcinoma (PDAC) is identifying new techniques to tailor gemcitabine and 5-fluorouracil treatments. Availability of tumor tissue is critical for the accurate assessment of gene expression, and laser microdissection (LMD) and primary cell cultures may be useful tools to separate tumor cells from the stromal reaction. The aim of this study was (1) to address the genetic profile relevant to drug activity and (2) to evaluate differences between microdissected and non-microdissected tumors, normal tissues, and primary cell cultures. Quantitative PCR of seven key genes was performed on mRNA from 113 microdissected and 28 non-microdissected tumors, a pool of normal tissues and four established primary cell lines. Protein expression was evaluated by western blot and immunocytochemistry and cytotoxicity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. LMD allowed the analysis of 110 samples and revealed significant differences in mRNA levels between microdissected tumors and normal tissues, as well as between non-microdissected and microdissected tumors from the same patients. In contrast, primary cell lines showed similar expression profiles with respect to their respective microdissected tumors. In particular, expression levels of human equilibrative nucleoside transporter-1 and thymydilate synthase were significantly related to gemcitabine and 5-fluorouracil cytotoxicity. We conclude that LMD is a reliable technique for mRNA extraction, and allows detection of significant differences in the expression of specific target genes when compared to non-microdissected specimens and normal tissues. Moreover, expression levels in microdissected tumors are similar to those observed in primary tumor cell cultures, both at mRNA and protein level, and are related to drug chemosensitivity. The use of these ex vivo techniques for molecular analysis of tumors therefore appears to be of some value in implementing the clinical management of PDAC." @default.
- W1968549005 created "2016-06-24" @default.
- W1968549005 creator A5019570497 @default.
- W1968549005 creator A5035992792 @default.
- W1968549005 creator A5036079877 @default.
- W1968549005 creator A5038421689 @default.
- W1968549005 creator A5041229832 @default.
- W1968549005 creator A5043782988 @default.
- W1968549005 creator A5048174487 @default.
- W1968549005 creator A5049269121 @default.
- W1968549005 creator A5049957859 @default.
- W1968549005 creator A5072567218 @default.
- W1968549005 creator A5077498316 @default.
- W1968549005 creator A5083693387 @default.
- W1968549005 creator A5086438669 @default.
- W1968549005 date "2008-07-01" @default.
- W1968549005 modified "2023-10-01" @default.
- W1968549005 title "Laser microdissection and primary cell cultures improve pharmacogenetic analysis in pancreatic adenocarcinoma" @default.
- W1968549005 cites W1931508686 @default.
- W1968549005 cites W1965734230 @default.
- W1968549005 cites W1973854724 @default.
- W1968549005 cites W1975004250 @default.
- W1968549005 cites W1978876168 @default.
- W1968549005 cites W1985585396 @default.
- W1968549005 cites W2023210471 @default.
- W1968549005 cites W2054829814 @default.
- W1968549005 cites W2054847513 @default.
- W1968549005 cites W2055142365 @default.
- W1968549005 cites W2061797905 @default.
- W1968549005 cites W2065488194 @default.
- W1968549005 cites W2077548774 @default.
- W1968549005 cites W2081419234 @default.
- W1968549005 cites W2103459879 @default.
- W1968549005 cites W2105202429 @default.
- W1968549005 cites W2107015211 @default.
- W1968549005 cites W2114787622 @default.
- W1968549005 cites W2118513443 @default.
- W1968549005 cites W2124059811 @default.
- W1968549005 cites W2127497086 @default.
- W1968549005 cites W2131025996 @default.
- W1968549005 cites W2131555383 @default.
- W1968549005 cites W2133896127 @default.
- W1968549005 cites W2140228520 @default.
- W1968549005 cites W2146616032 @default.
- W1968549005 cites W2153833969 @default.
- W1968549005 cites W2159122128 @default.
- W1968549005 cites W2161769699 @default.
- W1968549005 cites W2163608188 @default.
- W1968549005 cites W2164529446 @default.
- W1968549005 cites W2313253960 @default.
- W1968549005 cites W2324984848 @default.
- W1968549005 cites W4247785462 @default.
- W1968549005 doi "https://doi.org/10.1038/labinvest.2008.40" @default.
- W1968549005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18490900" @default.
- W1968549005 hasPublicationYear "2008" @default.
- W1968549005 type Work @default.
- W1968549005 sameAs 1968549005 @default.
- W1968549005 citedByCount "32" @default.
- W1968549005 countsByYear W19685490052012 @default.
- W1968549005 countsByYear W19685490052013 @default.
- W1968549005 countsByYear W19685490052014 @default.
- W1968549005 countsByYear W19685490052015 @default.
- W1968549005 countsByYear W19685490052016 @default.
- W1968549005 countsByYear W19685490052017 @default.
- W1968549005 countsByYear W19685490052018 @default.
- W1968549005 countsByYear W19685490052019 @default.
- W1968549005 countsByYear W19685490052020 @default.
- W1968549005 countsByYear W19685490052021 @default.
- W1968549005 countsByYear W19685490052022 @default.
- W1968549005 countsByYear W19685490052023 @default.
- W1968549005 crossrefType "journal-article" @default.
- W1968549005 hasAuthorship W1968549005A5019570497 @default.
- W1968549005 hasAuthorship W1968549005A5035992792 @default.
- W1968549005 hasAuthorship W1968549005A5036079877 @default.
- W1968549005 hasAuthorship W1968549005A5038421689 @default.
- W1968549005 hasAuthorship W1968549005A5041229832 @default.
- W1968549005 hasAuthorship W1968549005A5043782988 @default.
- W1968549005 hasAuthorship W1968549005A5048174487 @default.
- W1968549005 hasAuthorship W1968549005A5049269121 @default.
- W1968549005 hasAuthorship W1968549005A5049957859 @default.
- W1968549005 hasAuthorship W1968549005A5072567218 @default.
- W1968549005 hasAuthorship W1968549005A5077498316 @default.
- W1968549005 hasAuthorship W1968549005A5083693387 @default.
- W1968549005 hasAuthorship W1968549005A5086438669 @default.
- W1968549005 hasBestOaLocation W19685490051 @default.
- W1968549005 hasConcept C104317684 @default.
- W1968549005 hasConcept C11242284 @default.
- W1968549005 hasConcept C121608353 @default.
- W1968549005 hasConcept C125965507 @default.
- W1968549005 hasConcept C134018914 @default.
- W1968549005 hasConcept C142724271 @default.
- W1968549005 hasConcept C150194340 @default.
- W1968549005 hasConcept C153911025 @default.
- W1968549005 hasConcept C16930146 @default.
- W1968549005 hasConcept C2776737053 @default.
- W1968549005 hasConcept C2778764654 @default.
- W1968549005 hasConcept C2781182431 @default.
- W1968549005 hasConcept C35866371 @default.