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• W1968551526 startingPage "2349" @default.
• W1968551526 abstract "A method for RNA amplification in an immobilized medium is described. The medium contains a complete set of nucleotide substrates and purified Q beta replicase, an enzyme capable of exponentially amplifying RNAs under isothermal conditions. RNA amplification in the immobilized medium results in the formation of separate 'colonies', each comprising the progeny of a single RNA molecule (a clone). The colonies were visualized by staining with ethidium bromide, by utilizing radioactive substrates, and by hybridization with sequence-specific labeled probes. The number and identity of the RNA colonies corresponded to that of the RNAs seeded. When a mixture of different RNA species was seeded, these species were found in different colonies. Possible implementations of this technique include a search for recombinant RNAs, very sensitive nucleic acid diagnostics, and gene cloning in vitro." @default.
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• W1968551526 date "1993-01-01" @default.
• W1968551526 modified "2023-09-23" @default.
• W1968551526 title "Cloning of RNA molecules<i>in vitro</i>" @default.
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• W1968551526 doi "https://doi.org/10.1093/nar/21.10.2349" @default.
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