FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968561681> ?p ?o ?g. }

• W1968561681 endingPage "14055" @default.
• W1968561681 startingPage "14044" @default.
• W1968561681 abstract "EPR-silent, chemically reduced protocatechuate 3,4-dioxygenase (Er) binds NO at the active site Fe2+ to yield an EPR-active, S = 3/2 species that blocks subsequent binding of all other exogenous ligands. In contrast, addition of NO to a preformed Er·CN- complex yields an EPR-active, S = 1/2 species [Er·(CN)x·NO] that exhibits resolved superhyperfine splitting from 13CN-, 15/14NO, and a protein-derived 14N. Simulations of the EPR spectra observed for the Er·(CN)x·NO complex formed with 12CN- and 13CN- (1:1) show that CN- binds in two iron ligand sites (x ≥ 2). The two cyanides exhibit similar, but distinguishable, hyperfine coupling constants. This demonstrates unambiguously that at least three exogenous ligands (two cyanides and NO) can bind to the Fe2+ simultaneously and strongly suggests that at least one histidine ligand is retained in the complex. The Er·(CN)≥2·NO complex readily exchanges both of the bound cyanides for the substrate analog, 2-hydroxyisonicotinic acid N-oxide (INO), to form a Er·INO·NO complex exhibiting the same S = 3/2 type EPR spectrum that is observed for this complex in the absence of CN-. Because the dead-end Er·NO complex does not accumulate during the exchange, the results suggest that Er·(CN)≥2·NO and Er·INO·NO are in conformational states that allow facile exchange of INO and CN- but not NO. The results are interpreted in the context of the known X-ray crystal structures for the ferric form of the resting enzyme (Eox) and numerous Eox·substrate, inhibitor, and CN- complexes, all of which have a charge neutral iron center. It is proposed that the binding of one CN- causes dissociation of an anionic endogenous ligand which begins a series of conformational changes analogous to those initiated by anionic substrate binding to Eox. This results in a new unique coordination site for NO, and a new second site for CN-; both cyanide sites are utilized when the enzyme subsequently binds substrates or INO." @default.
• W1968561681 created "2016-06-24" @default.
• W1968561681 creator A5019917073 @default.
• W1968561681 creator A5053214968 @default.
• W1968561681 date "1997-11-01" @default.
• W1968561681 modified "2023-09-27" @default.
• W1968561681 title "Cyanide and Nitric Oxide Binding to Reduced Protocatechuate 3,4-Dioxygenase: Insight into the Basis for Order-Dependent Ligand Binding by Intradiol Catecholic Dioxygenases" @default.
• W1968561681 cites W1492352549 @default.
• W1968561681 cites W1509366823 @default.
• W1968561681 cites W1551004756 @default.
• W1968561681 cites W1559495651 @default.
• W1968561681 cites W1565948699 @default.
• W1968561681 cites W1575373997 @default.
• W1968561681 cites W1967445641 @default.
• W1968561681 cites W1972796256 @default.
• W1968561681 cites W1975968619 @default.
• W1968561681 cites W2042059875 @default.
• W1968561681 cites W2068254002 @default.
• W1968561681 cites W2068625006 @default.
• W1968561681 cites W2082040139 @default.
• W1968561681 cites W2086855564 @default.
• W1968561681 cites W2095081897 @default.
• W1968561681 cites W2153994323 @default.
• W1968561681 cites W3005098542 @default.
• W1968561681 doi "https://doi.org/10.1021/bi970609l" @default.
• W1968561681 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9369476" @default.
• W1968561681 hasPublicationYear "1997" @default.
• W1968561681 type Work @default.
• W1968561681 sameAs 1968561681 @default.
• W1968561681 citedByCount "22" @default.
• W1968561681 countsByYear W19685616812012 @default.
• W1968561681 countsByYear W19685616812016 @default.
• W1968561681 countsByYear W19685616812022 @default.
• W1968561681 countsByYear W19685616812023 @default.
• W1968561681 crossrefType "journal-article" @default.
• W1968561681 hasAuthorship W1968561681A5019917073 @default.
• W1968561681 hasAuthorship W1968561681A5053214968 @default.
• W1968561681 hasConcept C107824862 @default.
• W1968561681 hasConcept C116569031 @default.
• W1968561681 hasConcept C121332964 @default.
• W1968561681 hasConcept C126838900 @default.
• W1968561681 hasConcept C134121241 @default.
• W1968561681 hasConcept C143409427 @default.
• W1968561681 hasConcept C151730666 @default.
• W1968561681 hasConcept C170493617 @default.
• W1968561681 hasConcept C178790620 @default.
• W1968561681 hasConcept C179104552 @default.
• W1968561681 hasConcept C181199279 @default.
• W1968561681 hasConcept C185592680 @default.
• W1968561681 hasConcept C187961010 @default.
• W1968561681 hasConcept C191897082 @default.
• W1968561681 hasConcept C192562407 @default.
• W1968561681 hasConcept C193812863 @default.
• W1968561681 hasConcept C2776217839 @default.
• W1968561681 hasConcept C2776834422 @default.
• W1968561681 hasConcept C2778455166 @default.
• W1968561681 hasConcept C2779343474 @default.
• W1968561681 hasConcept C41183919 @default.
• W1968561681 hasConcept C46141821 @default.
• W1968561681 hasConcept C55493867 @default.
• W1968561681 hasConcept C71240020 @default.
• W1968561681 hasConcept C71924100 @default.
• W1968561681 hasConcept C75473681 @default.
• W1968561681 hasConcept C8010536 @default.
• W1968561681 hasConcept C83042744 @default.
• W1968561681 hasConcept C86803240 @default.
• W1968561681 hasConceptScore W1968561681C107824862 @default.
• W1968561681 hasConceptScore W1968561681C116569031 @default.
• W1968561681 hasConceptScore W1968561681C121332964 @default.
• W1968561681 hasConceptScore W1968561681C126838900 @default.
• W1968561681 hasConceptScore W1968561681C134121241 @default.
• W1968561681 hasConceptScore W1968561681C143409427 @default.
• W1968561681 hasConceptScore W1968561681C151730666 @default.
• W1968561681 hasConceptScore W1968561681C170493617 @default.
• W1968561681 hasConceptScore W1968561681C178790620 @default.
• W1968561681 hasConceptScore W1968561681C179104552 @default.
• W1968561681 hasConceptScore W1968561681C181199279 @default.
• W1968561681 hasConceptScore W1968561681C185592680 @default.
• W1968561681 hasConceptScore W1968561681C187961010 @default.
• W1968561681 hasConceptScore W1968561681C191897082 @default.
• W1968561681 hasConceptScore W1968561681C192562407 @default.
• W1968561681 hasConceptScore W1968561681C193812863 @default.
• W1968561681 hasConceptScore W1968561681C2776217839 @default.
• W1968561681 hasConceptScore W1968561681C2776834422 @default.
• W1968561681 hasConceptScore W1968561681C2778455166 @default.
• W1968561681 hasConceptScore W1968561681C2779343474 @default.
• W1968561681 hasConceptScore W1968561681C41183919 @default.
• W1968561681 hasConceptScore W1968561681C46141821 @default.
• W1968561681 hasConceptScore W1968561681C55493867 @default.
• W1968561681 hasConceptScore W1968561681C71240020 @default.
• W1968561681 hasConceptScore W1968561681C71924100 @default.
• W1968561681 hasConceptScore W1968561681C75473681 @default.
• W1968561681 hasConceptScore W1968561681C8010536 @default.
• W1968561681 hasConceptScore W1968561681C83042744 @default.
• W1968561681 hasConceptScore W1968561681C86803240 @default.
• W1968561681 hasIssue "46" @default.
• W1968561681 hasLocation W19685616811 @default.
• W1968561681 hasLocation W19685616812 @default.

• next