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- W1968593808 abstract "Making use of 125I-labelled monoclonal rat antibodies against mouse Fc receptor we have developed a solid-phase radioimmunoassay for cell-free antigenic mouse Fc receptor (FcR). An 80% acetone solution was used for fixation of relatively large amounts of soluble proteins on PVC microtiter plates. As a result of this treatment FcR practically lost its activity to bind the Fc portion of an IgG molecule but retained its antigenicity, the binding of specific anti-FcR (aFcR) antibody being increased following acetone fixation. Concentrations of cell-free FcR in NP-40 extracts of FcR-expressing cells were calculated from the linear part of a standard curve and expressed in units of antigenic activity, 1 unit being the amount of antigenic FcR capable of binding 1 microgram of 125I-aFcR. The method may be used for detecting cell-free FcR as a minor constituent in a mixture of proteins." @default.
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- W1968593808 date "1984-03-01" @default.
- W1968593808 modified "2023-09-26" @default.
- W1968593808 title "A radioimmunoassay with monoclonal antibodies for the detection of antigenic cell-free Fc receptor" @default.
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- W1968593808 doi "https://doi.org/10.1016/0022-1759(84)90158-3" @default.
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