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• W1968596103 abstract "In this paper, we combine the advantages of aptamer, nanomaterial and antibody to design an electrochemical sandwich immunoassay for the ultrasensitive detection of human immunoglobulin E (IgE) by using methylene blue (MB) as electrochemical indicator. The sandwich structure is fabricated by using goat anti-human IgE as capturing probe. Aptamer-Au nanoparticles (NPs) conjugates are used both as a sandwich amplification element as well as an accumulation reagent of MB. Once the aptamer-Au NPs conjugates specifically bind to electrode surface, MB molecules are accumulated on its surface by the specific interaction of MB with G base of aptamer-Au NPs conjugates. Therefore, with the increase of human IgE concentration, more aptamer-Au-NPs conjugates are bound, and thus, more MB molecules are accumulated. A good linear relationship is obtained for the detection of human IgE over a range of 1–10,000 ng/ml with a lowest detection limit of 0.52 ng/ml. In addition, by using BSA, human IgA and human IgM as contrast, the excellent specificity of this sensing system for the detection of human IgE is also demonstrated." @default.
• W1968596103 created "2016-06-24" @default.
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• W1968596103 date "2010-04-15" @default.
• W1968596103 modified "2023-10-03" @default.
• W1968596103 title "Aptamer-Au NPs conjugates-accumulated methylene blue for the sensitive electrochemical immunoassay of protein" @default.
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• W1968596103 doi "https://doi.org/10.1016/j.talanta.2009.11.035" @default.
• W1968596103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20188888" @default.
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