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• W1968597797 abstract "We developed a novel type of real-time polymerase chain reaction (PCR) array with TaqMan chemistry as a platform for the comprehensive and semiquantitative detection of genetically modified (GM) crops. Thirty primer-probe sets for the specific detection of GM lines, recombinant DNA (r-DNA) segments, endogenous reference genes, and donor organisms were synthesized, and a 96-well PCR plate was prepared with a different primer-probe in each well as the real-time PCR array. The specificity and sensitivity of the array were evaluated. A comparative analysis with the data and publicly available information on GM crops approved in Japan allowed us to assume the possibility of unapproved GM crop contamination. Furthermore, we designed a Microsoft Excel spreadsheet application, Unapproved GMO Checker version 2.01, which helps process all the data of real-time PCR arrays for the easy assumption of unapproved GM crop contamination. The spreadsheet is available free of charge at http://cse.naro.affrc.go.jp/jmano/index.html." @default.
• W1968597797 created "2016-06-24" @default.
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• W1968597797 date "2008-12-12" @default.
• W1968597797 modified "2023-09-25" @default.
• W1968597797 title "Real-Time PCR Array as a Universal Platform for the Detection of Genetically Modified Crops and Its Application in Identifying Unapproved Genetically Modified Crops in Japan" @default.
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• W1968597797 doi "https://doi.org/10.1021/jf802551h" @default.
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