FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968602937> ?p ?o ?g. }

• W1968602937 endingPage "15140" @default.
• W1968602937 startingPage "15135" @default.
• W1968602937 abstract "The increasing sophistication of synthetic biology is creating a demand for robust, broadly accessible methodology for constructing multigene pathways inside of the cell. Due to the difficulty of rationally designing pathways that function as desired in vivo, there is a further need to assemble libraries of pathways in parallel, in order to facilitate the combinatorial optimization of performance. While some in vitro DNA assembly methods can theoretically make libraries of pathways, these techniques are resource intensive and inherently require additional techniques to move the DNA back into cells. All previously reported in vivo assembly techniques have been low yielding, generating only tens to hundreds of constructs at a time. Here, we develop Reiterative Recombination, a robust method for building multigene pathways directly in the yeast chromosome. Due to its use of endonuclease-induced homologous recombination in conjunction with recyclable markers, Reiterative Recombination provides a highly efficient, technically simple strategy for sequentially assembling an indefinite number of DNA constructs at a defined locus. In this work, we describe the design and construction of the first Reiterative Recombination system in Saccharomyces cerevisiae, and we show that it can be used to assemble multigene constructs. We further demonstrate that Reiterative Recombination can construct large mock libraries of at least 10(4) biosynthetic pathways. We anticipate that our system's simplicity and high efficiency will make it a broadly accessible technology for pathway construction and render it a valuable tool for optimizing pathways in vivo." @default.
• W1968602937 created "2016-06-24" @default.
• W1968602937 creator A5051614796 @default.
• W1968602937 creator A5081800434 @default.
• W1968602937 date "2011-08-26" @default.
• W1968602937 modified "2023-10-18" @default.
• W1968602937 title "Reiterative Recombination for the in vivo assembly of libraries of multigene pathways" @default.
• W1968602937 cites W1598985695 @default.
• W1968602937 cites W1967210731 @default.
• W1968602937 cites W1970893992 @default.
• W1968602937 cites W1971439989 @default.
• W1968602937 cites W1973335730 @default.
• W1968602937 cites W1976019826 @default.
• W1968602937 cites W1979159575 @default.
• W1968602937 cites W1981740353 @default.
• W1968602937 cites W1984248485 @default.
• W1968602937 cites W1990030711 @default.
• W1968602937 cites W1996568337 @default.
• W1968602937 cites W1998853911 @default.
• W1968602937 cites W2008402376 @default.
• W1968602937 cites W2017621033 @default.
• W1968602937 cites W2022186026 @default.
• W1968602937 cites W2022968407 @default.
• W1968602937 cites W2025972514 @default.
• W1968602937 cites W2029943478 @default.
• W1968602937 cites W2034717680 @default.
• W1968602937 cites W2039295326 @default.
• W1968602937 cites W2041250319 @default.
• W1968602937 cites W2041985017 @default.
• W1968602937 cites W2046578912 @default.
• W1968602937 cites W2059223148 @default.
• W1968602937 cites W2059464705 @default.
• W1968602937 cites W2061725821 @default.
• W1968602937 cites W2062736124 @default.
• W1968602937 cites W2063887472 @default.
• W1968602937 cites W2068344177 @default.
• W1968602937 cites W2079347688 @default.
• W1968602937 cites W2085980948 @default.
• W1968602937 cites W2086527676 @default.
• W1968602937 cites W2092313455 @default.
• W1968602937 cites W2095923527 @default.
• W1968602937 cites W2098305228 @default.
• W1968602937 cites W2102440675 @default.
• W1968602937 cites W2107409328 @default.
• W1968602937 cites W2134370272 @default.
• W1968602937 cites W2134783465 @default.
• W1968602937 cites W2137679210 @default.
• W1968602937 cites W2140443340 @default.
• W1968602937 cites W2144779133 @default.
• W1968602937 cites W2148924797 @default.
• W1968602937 cites W2150665661 @default.
• W1968602937 cites W2154797402 @default.
• W1968602937 cites W2158571208 @default.
• W1968602937 cites W2163052215 @default.
• W1968602937 cites W4211152707 @default.
• W1968602937 cites W4254536048 @default.
• W1968602937 cites W51967180 @default.
• W1968602937 doi "https://doi.org/10.1073/pnas.1100507108" @default.
• W1968602937 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3174608" @default.
• W1968602937 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21876185" @default.
• W1968602937 hasPublicationYear "2011" @default.
• W1968602937 type Work @default.
• W1968602937 sameAs 1968602937 @default.
• W1968602937 citedByCount "96" @default.
• W1968602937 countsByYear W19686029372012 @default.
• W1968602937 countsByYear W19686029372013 @default.
• W1968602937 countsByYear W19686029372014 @default.
• W1968602937 countsByYear W19686029372015 @default.
• W1968602937 countsByYear W19686029372016 @default.
• W1968602937 countsByYear W19686029372017 @default.
• W1968602937 countsByYear W19686029372018 @default.
• W1968602937 countsByYear W19686029372019 @default.
• W1968602937 countsByYear W19686029372020 @default.
• W1968602937 countsByYear W19686029372021 @default.
• W1968602937 countsByYear W19686029372022 @default.
• W1968602937 countsByYear W19686029372023 @default.
• W1968602937 crossrefType "journal-article" @default.
• W1968602937 hasAuthorship W1968602937A5051614796 @default.
• W1968602937 hasAuthorship W1968602937A5081800434 @default.
• W1968602937 hasBestOaLocation W19686029371 @default.
• W1968602937 hasConcept C102744134 @default.
• W1968602937 hasConcept C104317684 @default.
• W1968602937 hasConcept C156695909 @default.
• W1968602937 hasConcept C191908910 @default.
• W1968602937 hasConcept C2777576037 @default.
• W1968602937 hasConcept C41008148 @default.
• W1968602937 hasConcept C54355233 @default.
• W1968602937 hasConcept C552990157 @default.
• W1968602937 hasConcept C70721500 @default.
• W1968602937 hasConcept C86803240 @default.
• W1968602937 hasConceptScore W1968602937C102744134 @default.
• W1968602937 hasConceptScore W1968602937C104317684 @default.
• W1968602937 hasConceptScore W1968602937C156695909 @default.
• W1968602937 hasConceptScore W1968602937C191908910 @default.
• W1968602937 hasConceptScore W1968602937C2777576037 @default.
• W1968602937 hasConceptScore W1968602937C41008148 @default.
• W1968602937 hasConceptScore W1968602937C54355233 @default.
• W1968602937 hasConceptScore W1968602937C552990157 @default.

• next