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• W1968619039 abstract "We examined a series of transformed cell lines resulting from transfer of the herpes simplex virus type 1 thymidine kinase gene to Ltk− cells by protoplast fusion gene transfer. We show that multiple copies of the transforming plasmid DNA, ranging from a minimum of two to greater than 20, were present in one or at most a few integration sites in each cell line. The TK+ phenotype was stable in five independent transformed cell lines after growth in nonselective medium for over a year. Transforming plasmid DNA was stable in one cell line containing from two to five copies after a year of growth in nonselective medium. In another cell line initially containing about 20 copies, the transforming DNA became rearranged soon after growth to mass culture, resulting in a decrease to two to five copies which then remained stably maintained. This suggests that TK+ transformants resulting from protoplast fusion are stable when the input DNA has integrated in a relatively low copy number." @default.
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• W1968619039 date "1984-03-01" @default.
• W1968619039 modified "2023-09-23" @default.
• W1968619039 title "Herpes simplex virus thymidine kinase gene is stably maintained and expressed in cells transformed by protoplast fusion" @default.
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• W1968619039 doi "https://doi.org/10.1007/bf01534902" @default.
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• W1968619039 hasPublicationYear "1984" @default.
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