Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968651981> ?p ?o ?g. }
- W1968651981 endingPage "6806" @default.
- W1968651981 startingPage "6799" @default.
- W1968651981 abstract "A conformationally restricted B cell epitope has been identified as a potential safe vaccine candidate from the major group A streptococcal virulence factor, the M protein. To maintain alpha-helical secondary structure, the minimal epitope is flanked with heterologous sequences to produce the chimeric vaccine candidate called J14. As a strategy toward developing an affordable multivalent GAS vaccine, we have expressed J14 recombinantly with a second GAS protective antigen H12 (rJ14H12). When administered to mice sub-cutaneously, the fusion protein stimulated a strong serum IgG response to the H12 component, but J14 was poorly immunogenic. To increase the immunogenicity of J14 when expressed with the model fusion partner, amino acid modifications were made to the initial recombinant J14 construct to produce rJJo. These changes stabilised the alpha-helical conformation of the recombinant antigen as assessed by circular dichroism. Mice immunised with rJJoH12, the fusion protein incorporating JJo, effectively stimulated a humoral response to both of the included antigens. These data support the feasibility of developing a multivalent vaccine incorporating the conformationally restricted protective antigen J14." @default.
- W1968651981 created "2016-06-24" @default.
- W1968651981 creator A5021247221 @default.
- W1968651981 creator A5025007734 @default.
- W1968651981 creator A5031740341 @default.
- W1968651981 creator A5031876902 @default.
- W1968651981 creator A5086405597 @default.
- W1968651981 date "2009-11-01" @default.
- W1968651981 modified "2023-10-17" @default.
- W1968651981 title "Structural optimisation of a conformational epitope improves antigenicity when expressed as a recombinant fusion protein" @default.
- W1968651981 cites W1554313068 @default.
- W1968651981 cites W1563981780 @default.
- W1968651981 cites W1658170585 @default.
- W1968651981 cites W1918246416 @default.
- W1968651981 cites W1967446440 @default.
- W1968651981 cites W1970037234 @default.
- W1968651981 cites W1976694791 @default.
- W1968651981 cites W1976820316 @default.
- W1968651981 cites W1978464971 @default.
- W1968651981 cites W1981799048 @default.
- W1968651981 cites W1998315492 @default.
- W1968651981 cites W2016162029 @default.
- W1968651981 cites W2022836144 @default.
- W1968651981 cites W2057243522 @default.
- W1968651981 cites W2057362537 @default.
- W1968651981 cites W2066557507 @default.
- W1968651981 cites W2066721154 @default.
- W1968651981 cites W2072445985 @default.
- W1968651981 cites W2080851686 @default.
- W1968651981 cites W2091129784 @default.
- W1968651981 cites W2092209213 @default.
- W1968651981 cites W2098823687 @default.
- W1968651981 cites W2099654832 @default.
- W1968651981 cites W2108147481 @default.
- W1968651981 cites W2123893523 @default.
- W1968651981 cites W2134661315 @default.
- W1968651981 cites W2140379612 @default.
- W1968651981 cites W2147011991 @default.
- W1968651981 cites W2155035342 @default.
- W1968651981 cites W2168727901 @default.
- W1968651981 cites W2170550455 @default.
- W1968651981 cites W2171780167 @default.
- W1968651981 doi "https://doi.org/10.1016/j.vaccine.2009.08.049" @default.
- W1968651981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19729086" @default.
- W1968651981 hasPublicationYear "2009" @default.
- W1968651981 type Work @default.
- W1968651981 sameAs 1968651981 @default.
- W1968651981 citedByCount "16" @default.
- W1968651981 countsByYear W19686519812012 @default.
- W1968651981 countsByYear W19686519812013 @default.
- W1968651981 countsByYear W19686519812014 @default.
- W1968651981 countsByYear W19686519812016 @default.
- W1968651981 countsByYear W19686519812020 @default.
- W1968651981 countsByYear W19686519812022 @default.
- W1968651981 crossrefType "journal-article" @default.
- W1968651981 hasAuthorship W1968651981A5021247221 @default.
- W1968651981 hasAuthorship W1968651981A5025007734 @default.
- W1968651981 hasAuthorship W1968651981A5031740341 @default.
- W1968651981 hasAuthorship W1968651981A5031876902 @default.
- W1968651981 hasAuthorship W1968651981A5086405597 @default.
- W1968651981 hasBestOaLocation W19686519812 @default.
- W1968651981 hasConcept C104317684 @default.
- W1968651981 hasConcept C123894998 @default.
- W1968651981 hasConcept C147483822 @default.
- W1968651981 hasConcept C159047783 @default.
- W1968651981 hasConcept C195616568 @default.
- W1968651981 hasConcept C203014093 @default.
- W1968651981 hasConcept C2779026604 @default.
- W1968651981 hasConcept C2780868878 @default.
- W1968651981 hasConcept C40767141 @default.
- W1968651981 hasConcept C55493867 @default.
- W1968651981 hasConcept C86803240 @default.
- W1968651981 hasConcept C89423630 @default.
- W1968651981 hasConceptScore W1968651981C104317684 @default.
- W1968651981 hasConceptScore W1968651981C123894998 @default.
- W1968651981 hasConceptScore W1968651981C147483822 @default.
- W1968651981 hasConceptScore W1968651981C159047783 @default.
- W1968651981 hasConceptScore W1968651981C195616568 @default.
- W1968651981 hasConceptScore W1968651981C203014093 @default.
- W1968651981 hasConceptScore W1968651981C2779026604 @default.
- W1968651981 hasConceptScore W1968651981C2780868878 @default.
- W1968651981 hasConceptScore W1968651981C40767141 @default.
- W1968651981 hasConceptScore W1968651981C55493867 @default.
- W1968651981 hasConceptScore W1968651981C86803240 @default.
- W1968651981 hasConceptScore W1968651981C89423630 @default.
- W1968651981 hasIssue "48" @default.
- W1968651981 hasLocation W19686519811 @default.
- W1968651981 hasLocation W19686519812 @default.
- W1968651981 hasLocation W19686519813 @default.
- W1968651981 hasOpenAccess W1968651981 @default.
- W1968651981 hasPrimaryLocation W19686519811 @default.
- W1968651981 hasRelatedWork W1980021022 @default.
- W1968651981 hasRelatedWork W2019183242 @default.
- W1968651981 hasRelatedWork W2047187826 @default.
- W1968651981 hasRelatedWork W2072925709 @default.
- W1968651981 hasRelatedWork W2078042295 @default.
- W1968651981 hasRelatedWork W2392243388 @default.
- W1968651981 hasRelatedWork W2399733012 @default.