Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968652868> ?p ?o ?g. }
- W1968652868 endingPage "79" @default.
- W1968652868 startingPage "73" @default.
- W1968652868 abstract "The present study demonstrates the comparative thermal, conformational and kinetic stabilities of the three closely related enzymes; the mesophilic yeast alcohol dehydrogenase (YADH), horse liver alcohol dehydrogenase (HLADH), and the extreme-thermophilic Thermoanaerobacter brockii alcohol dehydrogenase (TBADH). The mid-point unfolding temperatures for TBADH and HLADH were at least 10 °C and 6 °C higher, respectively, than that of YADH. When YADH was completely inactivated by thermal stress, the residual activities of HLADH and TBADH were 70% and 100%, respectively. The optimum temperature (Topt) activities of HLADH and TBADH were at least 40 °C and 55 °C higher, respectively, than that of YADH. Due to the higher rigidity of HLADH and TBADH, the enzymatic activation energies of HLADH and TBADH were higher than that of YADH. Geometric X-ray analysis indicated a comparatively higher coil (turn and loop) percentage in TBADH and HLADH than in YADH. Pairwise alignment for TBADH/HLADH exhibited a similarity score approximately 2.5-fold greater than that of the TBADH/YADH pair. Multiple alignments made with ClustalW revealed a higher number of conserved proline residues in the two most stable enzymes (HLADH/TBADH). These extra prolines tend to occur in surface loops and are likely to be responsible for the increased stability of TBADH and HLADH, by loop rigidification." @default.
- W1968652868 created "2016-06-24" @default.
- W1968652868 creator A5004697854 @default.
- W1968652868 creator A5016832677 @default.
- W1968652868 creator A5029361146 @default.
- W1968652868 creator A5052645132 @default.
- W1968652868 date "2009-08-01" @default.
- W1968652868 modified "2023-10-17" @default.
- W1968652868 title "Comparative thermostability of mesophilic and thermophilic alcohol dehydrogenases: Stability-determining roles of proline residues and loop conformations" @default.
- W1968652868 cites W1508745139 @default.
- W1968652868 cites W1541189253 @default.
- W1968652868 cites W1549709155 @default.
- W1968652868 cites W1565223565 @default.
- W1968652868 cites W1598075072 @default.
- W1968652868 cites W1970167335 @default.
- W1968652868 cites W1970558045 @default.
- W1968652868 cites W1972946273 @default.
- W1968652868 cites W1977877548 @default.
- W1968652868 cites W1978424680 @default.
- W1968652868 cites W1984163549 @default.
- W1968652868 cites W1991205473 @default.
- W1968652868 cites W1996423252 @default.
- W1968652868 cites W2000388754 @default.
- W1968652868 cites W2001210310 @default.
- W1968652868 cites W2015171280 @default.
- W1968652868 cites W2022081324 @default.
- W1968652868 cites W2028854152 @default.
- W1968652868 cites W2031778321 @default.
- W1968652868 cites W2033658542 @default.
- W1968652868 cites W2040567657 @default.
- W1968652868 cites W2040758408 @default.
- W1968652868 cites W2043162595 @default.
- W1968652868 cites W2043901868 @default.
- W1968652868 cites W2051419400 @default.
- W1968652868 cites W2053473804 @default.
- W1968652868 cites W2065995835 @default.
- W1968652868 cites W2069960592 @default.
- W1968652868 cites W2070326904 @default.
- W1968652868 cites W2073553628 @default.
- W1968652868 cites W2076842629 @default.
- W1968652868 cites W2079257101 @default.
- W1968652868 cites W2092124694 @default.
- W1968652868 cites W2092845657 @default.
- W1968652868 cites W2093759930 @default.
- W1968652868 cites W2095724872 @default.
- W1968652868 cites W2097674466 @default.
- W1968652868 cites W2099264016 @default.
- W1968652868 cites W2103318377 @default.
- W1968652868 cites W2103797225 @default.
- W1968652868 cites W2116006533 @default.
- W1968652868 cites W2119183658 @default.
- W1968652868 cites W2125097548 @default.
- W1968652868 cites W2131610468 @default.
- W1968652868 cites W2146778975 @default.
- W1968652868 cites W2163627794 @default.
- W1968652868 cites W2950903386 @default.
- W1968652868 doi "https://doi.org/10.1016/j.enzmictec.2009.04.007" @default.
- W1968652868 hasPublicationYear "2009" @default.
- W1968652868 type Work @default.
- W1968652868 sameAs 1968652868 @default.
- W1968652868 citedByCount "24" @default.
- W1968652868 countsByYear W19686528682012 @default.
- W1968652868 countsByYear W19686528682013 @default.
- W1968652868 countsByYear W19686528682014 @default.
- W1968652868 countsByYear W19686528682015 @default.
- W1968652868 countsByYear W19686528682016 @default.
- W1968652868 countsByYear W19686528682017 @default.
- W1968652868 countsByYear W19686528682018 @default.
- W1968652868 countsByYear W19686528682021 @default.
- W1968652868 countsByYear W19686528682022 @default.
- W1968652868 countsByYear W19686528682023 @default.
- W1968652868 crossrefType "journal-article" @default.
- W1968652868 hasAuthorship W1968652868A5004697854 @default.
- W1968652868 hasAuthorship W1968652868A5016832677 @default.
- W1968652868 hasAuthorship W1968652868A5029361146 @default.
- W1968652868 hasAuthorship W1968652868A5052645132 @default.
- W1968652868 hasConcept C178790620 @default.
- W1968652868 hasConcept C181199279 @default.
- W1968652868 hasConcept C185592680 @default.
- W1968652868 hasConcept C2777344606 @default.
- W1968652868 hasConcept C2781216036 @default.
- W1968652868 hasConcept C27911776 @default.
- W1968652868 hasConcept C33633552 @default.
- W1968652868 hasConcept C523546767 @default.
- W1968652868 hasConcept C54355233 @default.
- W1968652868 hasConcept C59061564 @default.
- W1968652868 hasConcept C63223902 @default.
- W1968652868 hasConcept C71240020 @default.
- W1968652868 hasConcept C86803240 @default.
- W1968652868 hasConceptScore W1968652868C178790620 @default.
- W1968652868 hasConceptScore W1968652868C181199279 @default.
- W1968652868 hasConceptScore W1968652868C185592680 @default.
- W1968652868 hasConceptScore W1968652868C2777344606 @default.
- W1968652868 hasConceptScore W1968652868C2781216036 @default.
- W1968652868 hasConceptScore W1968652868C27911776 @default.
- W1968652868 hasConceptScore W1968652868C33633552 @default.
- W1968652868 hasConceptScore W1968652868C523546767 @default.
- W1968652868 hasConceptScore W1968652868C54355233 @default.