FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968655038> ?p ?o ?g. }

• W1968655038 endingPage "9" @default.
• W1968655038 startingPage "3" @default.
• W1968655038 abstract "Metabolomics, i.e., the multiparallel analysis of metabolite changes occurring in a cell or an organism, has become feasible with the development of highly efficient mass spectroscopic technologies. Functional genomics as a standard tool helped to identify the function of many of the genes that encode important transporters and metabolic enzymes over the past few years. Advanced expression systems and analysis technologies made it possible to study the biochemical properties of the corresponding proteins in great detail. We begin to understand the biological functions of the gene products by systematic analysis of mutants using systematic PTGS/RNAi, knockout and TILLING approaches. However, one crucial set of data especially relevant in the case of multicellular organisms is lacking: the knowledge of the spatial and temporal profiles of metabolite levels at cellular and subcellular levels.We therefore developed genetically encoded nanosensors for several metabolites to provide a basic set of tools for the determination of cytosolic and subcellular metabolite levels in real time by using fluorescence microscopy.Prototypes of these sensors were successfully used in vitro and also in vivo, i.e., to measure sugar levels in fungal and animal cells.One of the future goals will be to expand the set of sensors to a wider spectrum of substrates by using the natural spectrum of periplasmic binding proteins from bacteria and by computational design of proteins with altered binding pockets in conjunction with mutagenesis. This toolbox can then be applied for four-dimensional imaging of cells and tissues to elucidate the spatial and temporal distribution of metabolites as a discovery tool in functional genomics, as a tool for high-throughput, high-content screening for drugs, to test metabolic models, and to analyze the interplay of cells in a tissue or organ." @default.
• W1968655038 created "2016-06-24" @default.
• W1968655038 creator A5005370327 @default.
• W1968655038 creator A5006908046 @default.
• W1968655038 creator A5010508565 @default.
• W1968655038 creator A5043281640 @default.
• W1968655038 creator A5054436322 @default.
• W1968655038 creator A5055578831 @default.
• W1968655038 creator A5057147724 @default.
• W1968655038 creator A5060199251 @default.
• W1968655038 creator A5078963967 @default.
• W1968655038 creator A5090590370 @default.
• W1968655038 date "2005-02-01" @default.
• W1968655038 modified "2023-10-12" @default.
• W1968655038 title "Genetically encoded sensors for metabolites" @default.
• W1968655038 cites W1467406035 @default.
• W1968655038 cites W1481557920 @default.
• W1968655038 cites W1525183361 @default.
• W1968655038 cites W1551309200 @default.
• W1968655038 cites W1557879301 @default.
• W1968655038 cites W1559065621 @default.
• W1968655038 cites W1580325799 @default.
• W1968655038 cites W1585377629 @default.
• W1968655038 cites W1594136944 @default.
• W1968655038 cites W1806555373 @default.
• W1968655038 cites W1963726292 @default.
• W1968655038 cites W1965205049 @default.
• W1968655038 cites W1965703132 @default.
• W1968655038 cites W1969540020 @default.
• W1968655038 cites W1970356951 @default.
• W1968655038 cites W1972158606 @default.
• W1968655038 cites W1976332939 @default.
• W1968655038 cites W1983020696 @default.
• W1968655038 cites W1984043202 @default.
• W1968655038 cites W1991685821 @default.
• W1968655038 cites W1992872970 @default.
• W1968655038 cites W1996635480 @default.
• W1968655038 cites W1997161797 @default.
• W1968655038 cites W1999361871 @default.
• W1968655038 cites W1999814206 @default.
• W1968655038 cites W2000475623 @default.
• W1968655038 cites W2001272842 @default.
• W1968655038 cites W2007811014 @default.
• W1968655038 cites W2008165198 @default.
• W1968655038 cites W2009451766 @default.
• W1968655038 cites W2011032800 @default.
• W1968655038 cites W2013151879 @default.
• W1968655038 cites W2013412105 @default.
• W1968655038 cites W201479413 @default.
• W1968655038 cites W2015153554 @default.
• W1968655038 cites W2016303617 @default.
• W1968655038 cites W2021237552 @default.
• W1968655038 cites W2036308708 @default.
• W1968655038 cites W2036502774 @default.
• W1968655038 cites W2037427470 @default.
• W1968655038 cites W2039210066 @default.
• W1968655038 cites W2056125201 @default.
• W1968655038 cites W2057522454 @default.
• W1968655038 cites W2064331689 @default.
• W1968655038 cites W2075706275 @default.
• W1968655038 cites W2091571247 @default.
• W1968655038 cites W2093913522 @default.
• W1968655038 cites W2094463363 @default.
• W1968655038 cites W2116146765 @default.
• W1968655038 cites W2136396782 @default.
• W1968655038 cites W2137484861 @default.
• W1968655038 cites W2138032051 @default.
• W1968655038 cites W2149983499 @default.
• W1968655038 cites W2150153332 @default.
• W1968655038 cites W2156715387 @default.
• W1968655038 cites W2413430215 @default.
• W1968655038 cites W271775295 @default.
• W1968655038 doi "https://doi.org/10.1002/cyto.a.20119" @default.
• W1968655038 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2752217" @default.
• W1968655038 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15688353" @default.
• W1968655038 hasPublicationYear "2005" @default.
• W1968655038 type Work @default.
• W1968655038 sameAs 1968655038 @default.
• W1968655038 citedByCount "54" @default.
• W1968655038 countsByYear W19686550382012 @default.
• W1968655038 countsByYear W19686550382013 @default.
• W1968655038 countsByYear W19686550382014 @default.
• W1968655038 countsByYear W19686550382015 @default.
• W1968655038 countsByYear W19686550382016 @default.
• W1968655038 countsByYear W19686550382017 @default.
• W1968655038 countsByYear W19686550382018 @default.
• W1968655038 countsByYear W19686550382019 @default.
• W1968655038 countsByYear W19686550382020 @default.
• W1968655038 countsByYear W19686550382022 @default.
• W1968655038 crossrefType "journal-article" @default.
• W1968655038 hasAuthorship W1968655038A5005370327 @default.
• W1968655038 hasAuthorship W1968655038A5006908046 @default.
• W1968655038 hasAuthorship W1968655038A5010508565 @default.
• W1968655038 hasAuthorship W1968655038A5043281640 @default.
• W1968655038 hasAuthorship W1968655038A5054436322 @default.
• W1968655038 hasAuthorship W1968655038A5055578831 @default.
• W1968655038 hasAuthorship W1968655038A5057147724 @default.
• W1968655038 hasAuthorship W1968655038A5060199251 @default.

• next