FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968655526> ?p ?o ?g. }

• W1968655526 endingPage "320" @default.
• W1968655526 startingPage "316" @default.
• W1968655526 abstract "The trabecular meshwork (TM) region of the eye is exposed to a constant low-level of oxidative insult. The cumulative damage may be the reason behind age-dependent risk for developing primary open angle glaucoma. Chronic and acute effects of hydrogen peroxide (H2O2) on TM endothelial cells include changes in viability, protein synthesis, and cellular adhesion. However, little if anything is known about the immediate effect of H2O2 on the biochemistry of the TM cells and the initial response to oxidative stress. In this report, we have used two-photon excitation autofluorescence (2PAF) to monitor changes to TM cell nicotinamide adenine dinucleotide (NADPH). 2PAF allows non-destructive, real-time analysis of concentration of intracellular NADPH. Coupled to reduced glutathione, NADPH, is a major component in the anti-oxidant defense of TM cells. Cultured human TM cells were monitored for over 30 min in control and H2O2-containing solutions. Peroxide caused both a dose- and time-dependent decrease in NADPH signal. NADPH fluorescence in control and in 4 mM H2O2 solutions showed little attenuation of NADPH signal (4% and 9% respectively). TM cell NADPH fluorescence showed a linear decrease with exposure to 20 mM H2O2 (−29%) and 100 mM H2O2 (37%) after a 30 min exposure. Exposure of TM cells to 500 mM H2O2 caused an exponential decrease in NADPH fluorescence to a final attenuation of 46% of starting intensity. Analysis of individual TM cells indicates that cells with higher initial NADPH fluorescence are more refractive to the apparent loss of viability caused by H2O2 than weakly fluorescing TM cells. We conclude that 2PAF of intracellular NADPH is a valuable tool for studying TM cell metabolism in response to oxidative insult." @default.
• W1968655526 created "2016-06-24" @default.
• W1968655526 creator A5000955825 @default.
• W1968655526 creator A5032412882 @default.
• W1968655526 creator A5052489228 @default.
• W1968655526 creator A5082800015 @default.
• W1968655526 creator A5088839478 @default.
• W1968655526 date "2011-09-01" @default.
• W1968655526 modified "2023-09-23" @default.
• W1968655526 title "Real-time measurements of nicotinamide adenine dinucleotide in live human trabecular meshwork cells: Effects of acute oxidative stress" @default.
• W1968655526 cites W1965780404 @default.
• W1968655526 cites W1969967464 @default.
• W1968655526 cites W1973020985 @default.
• W1968655526 cites W2043326394 @default.
• W1968655526 cites W2047453651 @default.
• W1968655526 cites W2051011515 @default.
• W1968655526 cites W2070828755 @default.
• W1968655526 cites W2077412169 @default.
• W1968655526 cites W2088402556 @default.
• W1968655526 cites W2096105943 @default.
• W1968655526 cites W2110310702 @default.
• W1968655526 doi "https://doi.org/10.1016/j.exer.2011.02.012" @default.
• W1968655526 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5042686" @default.
• W1968655526 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21354135" @default.
• W1968655526 hasPublicationYear "2011" @default.
• W1968655526 type Work @default.
• W1968655526 sameAs 1968655526 @default.
• W1968655526 citedByCount "13" @default.
• W1968655526 countsByYear W19686555262012 @default.
• W1968655526 countsByYear W19686555262013 @default.
• W1968655526 countsByYear W19686555262014 @default.
• W1968655526 countsByYear W19686555262017 @default.
• W1968655526 countsByYear W19686555262020 @default.
• W1968655526 countsByYear W19686555262022 @default.
• W1968655526 crossrefType "journal-article" @default.
• W1968655526 hasAuthorship W1968655526A5000955825 @default.
• W1968655526 hasAuthorship W1968655526A5032412882 @default.
• W1968655526 hasAuthorship W1968655526A5052489228 @default.
• W1968655526 hasAuthorship W1968655526A5082800015 @default.
• W1968655526 hasAuthorship W1968655526A5088839478 @default.
• W1968655526 hasBestOaLocation W19686555262 @default.
• W1968655526 hasConcept C12554922 @default.
• W1968655526 hasConcept C169760540 @default.
• W1968655526 hasConcept C181199279 @default.
• W1968655526 hasConcept C185592680 @default.
• W1968655526 hasConcept C197957613 @default.
• W1968655526 hasConcept C2775859393 @default.
• W1968655526 hasConcept C2776151105 @default.
• W1968655526 hasConcept C2776901170 @default.
• W1968655526 hasConcept C2777989768 @default.
• W1968655526 hasConcept C2778527774 @default.
• W1968655526 hasConcept C2781121420 @default.
• W1968655526 hasConcept C38485361 @default.
• W1968655526 hasConcept C48349386 @default.
• W1968655526 hasConcept C533411734 @default.
• W1968655526 hasConcept C538909803 @default.
• W1968655526 hasConcept C55493867 @default.
• W1968655526 hasConcept C57600042 @default.
• W1968655526 hasConcept C75520062 @default.
• W1968655526 hasConcept C86803240 @default.
• W1968655526 hasConceptScore W1968655526C12554922 @default.
• W1968655526 hasConceptScore W1968655526C169760540 @default.
• W1968655526 hasConceptScore W1968655526C181199279 @default.
• W1968655526 hasConceptScore W1968655526C185592680 @default.
• W1968655526 hasConceptScore W1968655526C197957613 @default.
• W1968655526 hasConceptScore W1968655526C2775859393 @default.
• W1968655526 hasConceptScore W1968655526C2776151105 @default.
• W1968655526 hasConceptScore W1968655526C2776901170 @default.
• W1968655526 hasConceptScore W1968655526C2777989768 @default.
• W1968655526 hasConceptScore W1968655526C2778527774 @default.
• W1968655526 hasConceptScore W1968655526C2781121420 @default.
• W1968655526 hasConceptScore W1968655526C38485361 @default.
• W1968655526 hasConceptScore W1968655526C48349386 @default.
• W1968655526 hasConceptScore W1968655526C533411734 @default.
• W1968655526 hasConceptScore W1968655526C538909803 @default.
• W1968655526 hasConceptScore W1968655526C55493867 @default.
• W1968655526 hasConceptScore W1968655526C57600042 @default.
• W1968655526 hasConceptScore W1968655526C75520062 @default.
• W1968655526 hasConceptScore W1968655526C86803240 @default.
• W1968655526 hasIssue "3" @default.
• W1968655526 hasLocation W19686555261 @default.
• W1968655526 hasLocation W19686555262 @default.
• W1968655526 hasLocation W19686555263 @default.
• W1968655526 hasLocation W19686555264 @default.
• W1968655526 hasOpenAccess W1968655526 @default.
• W1968655526 hasPrimaryLocation W19686555261 @default.
• W1968655526 hasRelatedWork W163228302 @default.
• W1968655526 hasRelatedWork W1982974506 @default.
• W1968655526 hasRelatedWork W2054970328 @default.
• W1968655526 hasRelatedWork W2093763987 @default.
• W1968655526 hasRelatedWork W2239781165 @default.
• W1968655526 hasRelatedWork W2320818839 @default.
• W1968655526 hasRelatedWork W3011891119 @default.
• W1968655526 hasRelatedWork W4205322090 @default.
• W1968655526 hasRelatedWork W68578887 @default.
• W1968655526 hasRelatedWork W2174342169 @default.
• W1968655526 hasVolume "93" @default.
• W1968655526 isParatext "false" @default.

• next