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- W1968656783 abstract "We describe the combination of a flexible multiphoton tomograph (MPTflex) with a heating and cooling stage. The stage allows temperature control in the range of (−196 °C) (77 K) to +600 °C (873 K) with selectable heating/freezing rates between 0.01 K min−1 and 150 K min−1. To illustrate the imaging capabilities of the combined system, fluorescence intensity and lifetime of intrinsic molecules from a plant leaf were imaged with submicron resolution during freezing in vivo without detaching the leaf from the plant. An increase of fluorescence intensity and decay times with decreasing temperature was observed. The measurements illustrate the usefulness of multiphoton imaging as a non-invasive online tool to investigate temperature-induced effects. The flexible multiphoton tomograph with its adjustable mechano-optical arm and scan head allows imaging at otherwise hardly accessible sample regions." @default.
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- W1968656783 date "2015-01-05" @default.
- W1968656783 modified "2023-09-27" @default.
- W1968656783 title "Two-photon imaging of intact living plants during freezing with a flexible multiphoton tomograph" @default.
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- W1968656783 doi "https://doi.org/10.1088/1612-2011/12/2/025601" @default.
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