FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968672291> ?p ?o ?g. }

• W1968672291 endingPage "1968" @default.
• W1968672291 startingPage "1962" @default.
• W1968672291 abstract "ABSTRACT De-esterification is an important degradation or detoxification mechanism of sulfonylurea herbicide in microbes and plants. However, the biochemical and molecular mechanisms of sulfonylurea herbicide de-esterification are still unknown. In this study, a novel esterase gene, sulE , responsible for sulfonylurea herbicide de-esterification, was cloned from Hansschlegelia zhihuaiae S113. The gene contained an open reading frame of 1,194 bp, and a putative signal peptide at the N terminal was identified with a predicted cleavage site between Ala37 and Glu38, resulting in a 361-residue mature protein. SulE minus the signal peptide was synthesized in Escherichia coli BL21 and purified to homogeneity. SulE catalyzed the de-esterification of a variety of sulfonylurea herbicides that gave rise to the corresponding herbicidally inactive parent acid and exhibited the highest catalytic efficiency toward thifensulfuron-methyl. SulE was a dimer without the requirement of a cofactor. The activity of the enzyme was completely inhibited by Ag + , Cd 2+ , Zn 2+ , methamidophos, and sodium dodecyl sulfate. A sulE -disrupted mutant strain, Δ sulE , was constructed by insertion mutation. Δ sulE lost the de-esterification ability and was more sensitive to the herbicides than the wild type of strain S113, suggesting that sulE played a vital role in the sulfonylurea herbicide resistance of the strain. The transfer of sulE into Saccharomyces cerevisiae BY4741 conferred on it the ability to de-esterify sulfonylurea herbicides and increased its resistance to the herbicides. This study has provided an excellent candidate for the mechanistic study of sulfonylurea herbicide metabolism and detoxification through de-esterification, construction of sulfonylurea herbicide-resistant transgenic crops, and bioremediation of sulfonylurea herbicide-contaminated environments." @default.
• W1968672291 created "2016-06-24" @default.
• W1968672291 creator A5004468414 @default.
• W1968672291 creator A5012145168 @default.
• W1968672291 creator A5033969008 @default.
• W1968672291 creator A5043980190 @default.
• W1968672291 creator A5057562112 @default.
• W1968672291 creator A5063813625 @default.
• W1968672291 creator A5083601595 @default.
• W1968672291 date "2012-03-15" @default.
• W1968672291 modified "2023-09-23" @default.
• W1968672291 title "SulE, a Sulfonylurea Herbicide De-Esterification Esterase from Hansschlegelia zhihuaiae S113" @default.
• W1968672291 cites W1507012231 @default.
• W1968672291 cites W1554763469 @default.
• W1968672291 cites W178547562 @default.
• W1968672291 cites W1961013080 @default.
• W1968672291 cites W1965656362 @default.
• W1968672291 cites W1973726278 @default.
• W1968672291 cites W1974248650 @default.
• W1968672291 cites W1980199994 @default.
• W1968672291 cites W1985705221 @default.
• W1968672291 cites W1986132384 @default.
• W1968672291 cites W1989327058 @default.
• W1968672291 cites W1990718823 @default.
• W1968672291 cites W2011258208 @default.
• W1968672291 cites W2013002648 @default.
• W1968672291 cites W2019121099 @default.
• W1968672291 cites W2022909207 @default.
• W1968672291 cites W2025479240 @default.
• W1968672291 cites W2040917634 @default.
• W1968672291 cites W2043672902 @default.
• W1968672291 cites W2045915009 @default.
• W1968672291 cites W2051813239 @default.
• W1968672291 cites W2060913542 @default.
• W1968672291 cites W2074887825 @default.
• W1968672291 cites W2075221933 @default.
• W1968672291 cites W2077616306 @default.
• W1968672291 cites W2097753075 @default.
• W1968672291 cites W2098492595 @default.
• W1968672291 cites W2100837269 @default.
• W1968672291 cites W2105137924 @default.
• W1968672291 cites W2109993282 @default.
• W1968672291 cites W2116137883 @default.
• W1968672291 cites W2121880975 @default.
• W1968672291 cites W2139151091 @default.
• W1968672291 cites W2143596568 @default.
• W1968672291 cites W2154893268 @default.
• W1968672291 cites W2157649587 @default.
• W1968672291 cites W2161746138 @default.
• W1968672291 cites W2163816147 @default.
• W1968672291 cites W2393737675 @default.
• W1968672291 cites W4293247451 @default.
• W1968672291 doi "https://doi.org/10.1128/aem.07440-11" @default.
• W1968672291 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3298168" @default.
• W1968672291 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22247165" @default.
• W1968672291 hasPublicationYear "2012" @default.
• W1968672291 type Work @default.
• W1968672291 sameAs 1968672291 @default.
• W1968672291 citedByCount "44" @default.
• W1968672291 countsByYear W19686722912012 @default.
• W1968672291 countsByYear W19686722912014 @default.
• W1968672291 countsByYear W19686722912015 @default.
• W1968672291 countsByYear W19686722912016 @default.
• W1968672291 countsByYear W19686722912018 @default.
• W1968672291 countsByYear W19686722912019 @default.
• W1968672291 countsByYear W19686722912020 @default.
• W1968672291 countsByYear W19686722912021 @default.
• W1968672291 countsByYear W19686722912022 @default.
• W1968672291 countsByYear W19686722912023 @default.
• W1968672291 crossrefType "journal-article" @default.
• W1968672291 hasAuthorship W1968672291A5004468414 @default.
• W1968672291 hasAuthorship W1968672291A5012145168 @default.
• W1968672291 hasAuthorship W1968672291A5033969008 @default.
• W1968672291 hasAuthorship W1968672291A5043980190 @default.
• W1968672291 hasAuthorship W1968672291A5057562112 @default.
• W1968672291 hasAuthorship W1968672291A5063813625 @default.
• W1968672291 hasAuthorship W1968672291A5083601595 @default.
• W1968672291 hasBestOaLocation W19686722911 @default.
• W1968672291 hasConcept C104317684 @default.
• W1968672291 hasConcept C143065580 @default.
• W1968672291 hasConcept C150903083 @default.
• W1968672291 hasConcept C181199279 @default.
• W1968672291 hasConcept C185592680 @default.
• W1968672291 hasConcept C2777261171 @default.
• W1968672291 hasConcept C2778854520 @default.
• W1968672291 hasConcept C2779222958 @default.
• W1968672291 hasConcept C2779306644 @default.
• W1968672291 hasConcept C55493867 @default.
• W1968672291 hasConcept C86803240 @default.
• W1968672291 hasConceptScore W1968672291C104317684 @default.
• W1968672291 hasConceptScore W1968672291C143065580 @default.
• W1968672291 hasConceptScore W1968672291C150903083 @default.
• W1968672291 hasConceptScore W1968672291C181199279 @default.
• W1968672291 hasConceptScore W1968672291C185592680 @default.
• W1968672291 hasConceptScore W1968672291C2777261171 @default.
• W1968672291 hasConceptScore W1968672291C2778854520 @default.
• W1968672291 hasConceptScore W1968672291C2779222958 @default.
• W1968672291 hasConceptScore W1968672291C2779306644 @default.

• next