FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968699130> ?p ?o ?g. }

• W1968699130 endingPage "5165" @default.
• W1968699130 startingPage "5165" @default.
• W1968699130 abstract "To develop a protocol for direct hepatic lineage differentiation from early developmental progenitors to a population of mature hepatocytes.Hepatic progenitor cells and then mature hepatocytes from mouse embryonic stem (ES) cells were obtained in a sequential manner, induced by valproic acid (VPA) and cytokines (hepatocyte growth factor, epidermal growth factor and insulin). Morphological changes of the differentiated cells were examined by phase-contrast microscopy and electron microscopy. Reverse transcription polymerase chain reaction and immunocytochemical analyses were used to evaluate the gene expression profiles of the VPA-induced hepatic progenitors and the hepatic progenitor-derived hepatocytes. Glycogen storage, cytochrome P450 activity, transplantation assay, differentiation of bile duct-like structures and tumorigenic analyses were performed for the functional identification of the differentiated cells. Furthermore, FACS and electron microscopy were used for the analyses of cell cycle profile and apoptosis in VPA-induced hepatic differentiated cells.Based on the combination of VPA and cytokines, mouse ES cells differentiated into a uniform and homogeneous cell population of hepatic progenitor cells and then matured into functional hepatocytes. The progenitor population shared several characteristics with ES cells and hepatic stem/progenitor cells, and represented a novel progenitor cell between ES and hepatic oval cells in embryonic development. The differentiated hepatocytes from progenitor cells shared typical characteristics with mature hepatocytes, including the patterns of gene expression, immunological markers, in vitro hepatocyte functions and in vivo capacity to restore acute-damaged liver function. In addition, the differentiation of hepatic progenitor cells from ES cells was accompanied by significant cell cycle arrest and selective survival of differentiating cells towards hepatic lineages.Hepatic cells of different developmental stages from early progenitors to matured hepatocytes can be acquired in the appropriate order based on sequential induction with VPA and cytokines." @default.
• W1968699130 created "2016-06-24" @default.
• W1968699130 creator A5016095471 @default.
• W1968699130 creator A5018013761 @default.
• W1968699130 creator A5026620143 @default.
• W1968699130 creator A5029058502 @default.
• W1968699130 creator A5037001394 @default.
• W1968699130 creator A5037454238 @default.
• W1968699130 creator A5082747056 @default.
• W1968699130 date "2009-01-01" @default.
• W1968699130 modified "2023-09-25" @default.
• W1968699130 title "Direct hepatic differentiation of mouse embryonic stemcells induced by valproic acid and cytokines" @default.
• W1968699130 cites W1539041175 @default.
• W1968699130 cites W1966450565 @default.
• W1968699130 cites W1970023965 @default.
• W1968699130 cites W1972149587 @default.
• W1968699130 cites W1976662121 @default.
• W1968699130 cites W1982824678 @default.
• W1968699130 cites W1992043159 @default.
• W1968699130 cites W1992837470 @default.
• W1968699130 cites W1993111223 @default.
• W1968699130 cites W1997590330 @default.
• W1968699130 cites W1999914688 @default.
• W1968699130 cites W2004364243 @default.
• W1968699130 cites W2006009416 @default.
• W1968699130 cites W2015985384 @default.
• W1968699130 cites W2017865707 @default.
• W1968699130 cites W2022285476 @default.
• W1968699130 cites W2030648440 @default.
• W1968699130 cites W2032105048 @default.
• W1968699130 cites W2032346637 @default.
• W1968699130 cites W2038439057 @default.
• W1968699130 cites W2039307777 @default.
• W1968699130 cites W2040222339 @default.
• W1968699130 cites W2046104499 @default.
• W1968699130 cites W2061248120 @default.
• W1968699130 cites W2072375912 @default.
• W1968699130 cites W2074991881 @default.
• W1968699130 cites W2090800257 @default.
• W1968699130 cites W2095309562 @default.
• W1968699130 cites W2112246274 @default.
• W1968699130 cites W2163352549 @default.
• W1968699130 cites W2168589849 @default.
• W1968699130 cites W2169656634 @default.
• W1968699130 cites W2169687662 @default.
• W1968699130 doi "https://doi.org/10.3748/wjg.15.5165" @default.
• W1968699130 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2773895" @default.
• W1968699130 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19891015" @default.
• W1968699130 hasPublicationYear "2009" @default.
• W1968699130 type Work @default.
• W1968699130 sameAs 1968699130 @default.
• W1968699130 citedByCount "19" @default.
• W1968699130 countsByYear W19686991302012 @default.
• W1968699130 countsByYear W19686991302013 @default.
• W1968699130 countsByYear W19686991302014 @default.
• W1968699130 countsByYear W19686991302015 @default.
• W1968699130 countsByYear W19686991302016 @default.
• W1968699130 countsByYear W19686991302017 @default.
• W1968699130 countsByYear W19686991302020 @default.
• W1968699130 countsByYear W19686991302021 @default.
• W1968699130 countsByYear W19686991302022 @default.
• W1968699130 crossrefType "journal-article" @default.
• W1968699130 hasAuthorship W1968699130A5016095471 @default.
• W1968699130 hasAuthorship W1968699130A5018013761 @default.
• W1968699130 hasAuthorship W1968699130A5026620143 @default.
• W1968699130 hasAuthorship W1968699130A5029058502 @default.
• W1968699130 hasAuthorship W1968699130A5037001394 @default.
• W1968699130 hasAuthorship W1968699130A5037454238 @default.
• W1968699130 hasAuthorship W1968699130A5082747056 @default.
• W1968699130 hasBestOaLocation W19686991301 @default.
• W1968699130 hasConcept C104317684 @default.
• W1968699130 hasConcept C134018914 @default.
• W1968699130 hasConcept C144024400 @default.
• W1968699130 hasConcept C145103041 @default.
• W1968699130 hasConcept C148738053 @default.
• W1968699130 hasConcept C149923435 @default.
• W1968699130 hasConcept C153911025 @default.
• W1968699130 hasConcept C195521686 @default.
• W1968699130 hasConcept C201750760 @default.
• W1968699130 hasConcept C202751555 @default.
• W1968699130 hasConcept C20875687 @default.
• W1968699130 hasConcept C2776200302 @default.
• W1968699130 hasConcept C28328180 @default.
• W1968699130 hasConcept C2908647359 @default.
• W1968699130 hasConcept C2908801736 @default.
• W1968699130 hasConcept C55493867 @default.
• W1968699130 hasConcept C86803240 @default.
• W1968699130 hasConcept C95444343 @default.
• W1968699130 hasConceptScore W1968699130C104317684 @default.
• W1968699130 hasConceptScore W1968699130C134018914 @default.
• W1968699130 hasConceptScore W1968699130C144024400 @default.
• W1968699130 hasConceptScore W1968699130C145103041 @default.
• W1968699130 hasConceptScore W1968699130C148738053 @default.
• W1968699130 hasConceptScore W1968699130C149923435 @default.
• W1968699130 hasConceptScore W1968699130C153911025 @default.
• W1968699130 hasConceptScore W1968699130C195521686 @default.
• W1968699130 hasConceptScore W1968699130C201750760 @default.
• W1968699130 hasConceptScore W1968699130C202751555 @default.

• next