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- W1968774460 abstract "A simple bidirectional allele-specific PCR method is described for determining the -1082 A and G alleles in the interleukin-10 (IL-10) promoter region. This polymorphism is associated with IL-10 production capacity, and it is thus interesting to see whether different infectious and autoimmune conditions are associated with it. With our method, the A and G alleles may be studied simultaneously in a single PCR reaction, as amplification of the different alleles is performed by using 3'-mismatched and partly overlapping allele-specific upstream and downstream primers around the -1082 site. The fast and simple method described here is especially suitable for large-scale association studies." @default.
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- W1968774460 date "2001-01-01" @default.
- W1968774460 modified "2023-09-27" @default.
- W1968774460 title "Genotyping Interleukin-10 High and Low Producers with Single-Tube Bidirectional Allele-Specific Amplification" @default.
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- W1968774460 doi "https://doi.org/10.1159/000049184" @default.
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