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- W1968791845 abstract "Recently, attention is being focused on the application of molecular shuttle based on kinesin and microtubules. Essential point regarding molecular shuttles is the cargo-loading system. Since the intrinsic physiological mechanism underlying the selective binding of cargo to the tail domain of kinesin has not been clarified thus far, artificial binding systems have been utilized for cargo binding. For instance, in a previous study, biotin-avidin and antigen-antibody systems were used for cargo loading. These systems are known to be highly specific with extremely strong binding. However, they have a defect that the shuttle cannot release the cargoes. Therefore, it is required that the molecular shuttle should have the reversible cargo-loading function. In this study, we developed a new molecular shuttle system in which the cargoes of liposome labeled with M13 peptide reversibly binds to the shuttle of kinesin-CaM fusion protein in a Ca2+ concentration dependent manner. We prepared K560-CaM that consists of the N-terminal motor domain, neck region, α-helical coiled-coil region and CaM at the C-terminus. K560-CaM showed Ca2+ dependent reversible binding to M13-GFP. Liposome labeled with fluorescent probe was prepared and its stability as a cargo was examined. Also maleimide- conjugated liposome was prepared. We also designed and synthesized the M13 peptide that has a single cysteine at N-terminus. It is known that the peptide is combined with a maleimide on the surface of the liposome. Currently we are studying the Ca2+ dependent interaction between M13-liposime and K560-CaM." @default.
- W1968791845 created "2016-06-24" @default.
- W1968791845 creator A5010713538 @default.
- W1968791845 date "2012-01-01" @default.
- W1968791845 modified "2023-09-29" @default.
- W1968791845 title "Molecular Shuttle with Calcium Dependent Cargo-Loading System using Kinesin-Cam Fusion Protein and Liposome" @default.
- W1968791845 doi "https://doi.org/10.1016/j.bpj.2011.11.2026" @default.
- W1968791845 hasPublicationYear "2012" @default.
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