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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968804544> ?p ?o ?g. }

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• W1968804544 abstract "Novel non-blinking quantum dots (NBQDs) were utilized in three-dimensional super-localization, high-precision tracking applications under an automated scanning-angle total internal reflection fluorescence microscope (SA-TIRFM). NBQDs were randomly attached to stationary microtubules along the radial axis under gliding assay conditions. By automatically scanning through a wide range of incident angles with different evanescent-field layer thicknesses, the fluorescence intensity decay curves were obtained. Fit with theoretical decay functions, the absolute vertical positions were determined with sub-10-nm localization precision. The emission intensity profile of the NBQDs attached to kinesin-propelled microtubules was used to resolve the self-rotation of gliding microtubules within a small vertical distance of ∼50 nm. We demonstrate the applicability of NBQDs in high-precision fluorescence imaging experiments." @default.
• W1968804544 created "2016-06-24" @default.
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• W1968804544 date "2012-04-02" @default.
• W1968804544 modified "2023-09-25" @default.
• W1968804544 title "High-Precision Tracking with Non-blinking Quantum Dots Resolves Nanoscale Vertical Displacement" @default.
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• W1968804544 doi "https://doi.org/10.1021/ja301332t" @default.
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