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• W1968843471 abstract "The present work describes the construction and application of a new DNA biosensor for detection of aflatoxin M1. In order to immobilize a thiol-modified single stranded DNA (ss-HSDNA) probe that specifically bound aflatoxin M1, a self-assembled monolayer of cysteamine and gold nanoparticles on the SAM were prepared on gold electrodes, layer-by-layer. The assembly processes of cysteamine, gold nanoparticles, and ss-HSDNA were monitored with the help of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. K3[Fe(CN)6]/K4[Fe(CN)6] solution was used as a redox probe for electrochemical measurements. The biosensor provided a linear response to aflatoxin M1 over the concentration range of 1–14 ng/mL with a standard deviation of ±0.36 ng/mL. Finally, the biosensor was applied to a series of real milk samples." @default.
• W1968843471 created "2016-06-24" @default.
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• W1968843471 date "2011-05-01" @default.
• W1968843471 modified "2023-10-01" @default.
• W1968843471 title "Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles" @default.
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• W1968843471 doi "https://doi.org/10.1016/j.bios.2011.02.038" @default.
• W1968843471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21420290" @default.
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