FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968863488> ?p ?o ?g. }

• W1968863488 endingPage "e85452" @default.
• W1968863488 startingPage "e85452" @default.
• W1968863488 abstract "Background Designing an ideal vaccine against HIV-1 has been difficult due to enormous genetic variability as a result of high replication rate and lack of proofreading activity of reverse transcriptase leading to emergence of genetic variants and recombinants. Tat transactivates HIV-1 LTR, resulting in a remarkable increase in viral gene expression, and plays a vital role in pathogenesis. The aim of this study was to characterize the genetic variations of Tat exon-1 from HIV-1 infected patients from North India. Methods Genomic DNA was isolated from PBMCs and Tat exon-1 was PCR amplified with specific primers followed by cloning, sequencing and sequence analyses using bioinformatic tools for predicting HIV-1 subtypes, recombination events, conservation of domains and phosphorylation sites, and LTR transactivation by luciferase assay. Results Phylogenetic analysis of Tat exon-1 variants (n = 120) revealed sequence similarity with South African Tat C sequences and distinct geographical relationships were observed for B/C recombinants. Bootscan analysis of our variants showed 90% homology to Tat C and 10% to B/C recombinants with a precise breakpoint. Natural substitutions were observed with high allelic frequencies which may be beneficial for virus. High amino acid conservation was observed in Tat among Anti Retroviral Therapy (ART) recipients. Barring few changes, most of the functional domains, predicted motifs and phosphorylation sites were well conserved in most of Tat variants. dN/dS analysis revealed purifying selection, implying the importance of functional conservation of Tat exon-1. Our Indian Tat C variants and B/C recombinants showed differential LTR transactivation. Conclusions The possible role of Tat exon-1 variants in shaping the current HIV-1 epidemic in North India was highlighted. Natural substitutions across conserved functional domains were observed and provided evidence for the emergence of B/C recombinants within the ORF of Tat exon-1. These events are likely to have implications for viral pathogenesis and vaccine formulations." @default.
• W1968863488 created "2016-06-24" @default.
• W1968863488 creator A5004319273 @default.
• W1968863488 creator A5008962287 @default.
• W1968863488 creator A5032406604 @default.
• W1968863488 creator A5062092073 @default.
• W1968863488 creator A5080182852 @default.
• W1968863488 creator A5080423374 @default.
• W1968863488 date "2014-01-23" @default.
• W1968863488 modified "2023-10-05" @default.
• W1968863488 title "Molecular and Genetic Characterization of Natural HIV-1 Tat Exon-1 Variants from North India and Their Functional Implications" @default.
• W1968863488 cites W1479813612 @default.
• W1968863488 cites W1507407662 @default.
• W1968863488 cites W1514108981 @default.
• W1968863488 cites W1560943379 @default.
• W1968863488 cites W1679796753 @default.
• W1968863488 cites W1752109011 @default.
• W1968863488 cites W1966425882 @default.
• W1968863488 cites W1971592398 @default.
• W1968863488 cites W1982271582 @default.
• W1968863488 cites W1993846907 @default.
• W1968863488 cites W1994836622 @default.
• W1968863488 cites W1998421163 @default.
• W1968863488 cites W2001214740 @default.
• W1968863488 cites W2003011401 @default.
• W1968863488 cites W2004669329 @default.
• W1968863488 cites W2006977189 @default.
• W1968863488 cites W2011630381 @default.
• W1968863488 cites W2014124795 @default.
• W1968863488 cites W2021188590 @default.
• W1968863488 cites W2027204532 @default.
• W1968863488 cites W2028031376 @default.
• W1968863488 cites W2030017358 @default.
• W1968863488 cites W2035269897 @default.
• W1968863488 cites W2035660778 @default.
• W1968863488 cites W2035748338 @default.
• W1968863488 cites W2038163496 @default.
• W1968863488 cites W2052785715 @default.
• W1968863488 cites W2065042047 @default.
• W1968863488 cites W2065597780 @default.
• W1968863488 cites W2066591348 @default.
• W1968863488 cites W2068151486 @default.
• W1968863488 cites W2068420800 @default.
• W1968863488 cites W2072329519 @default.
• W1968863488 cites W2076892180 @default.
• W1968863488 cites W2078422370 @default.
• W1968863488 cites W2081539383 @default.
• W1968863488 cites W2081885109 @default.
• W1968863488 cites W2087772385 @default.
• W1968863488 cites W2089449840 @default.
• W1968863488 cites W2090084965 @default.
• W1968863488 cites W2091646572 @default.
• W1968863488 cites W2092618307 @default.
• W1968863488 cites W2095388743 @default.
• W1968863488 cites W2097810423 @default.
• W1968863488 cites W2104919701 @default.
• W1968863488 cites W2106448245 @default.
• W1968863488 cites W2106882534 @default.
• W1968863488 cites W2108463233 @default.
• W1968863488 cites W2109113807 @default.
• W1968863488 cites W2118539769 @default.
• W1968863488 cites W2121677860 @default.
• W1968863488 cites W2127284613 @default.
• W1968863488 cites W2132632499 @default.
• W1968863488 cites W2133842761 @default.
• W1968863488 cites W2134992605 @default.
• W1968863488 cites W2135048849 @default.
• W1968863488 cites W2151292912 @default.
• W1968863488 cites W2152640863 @default.
• W1968863488 cites W2154632774 @default.
• W1968863488 cites W2155809660 @default.
• W1968863488 cites W2158666214 @default.
• W1968863488 cites W2160806431 @default.
• W1968863488 cites W2161990493 @default.
• W1968863488 cites W2162891851 @default.
• W1968863488 cites W2163400707 @default.
• W1968863488 cites W2165194856 @default.
• W1968863488 doi "https://doi.org/10.1371/journal.pone.0085452" @default.
• W1968863488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3900424" @default.
• W1968863488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24465566" @default.
• W1968863488 hasPublicationYear "2014" @default.
• W1968863488 type Work @default.
• W1968863488 sameAs 1968863488 @default.
• W1968863488 citedByCount "17" @default.
• W1968863488 countsByYear W19688634882015 @default.
• W1968863488 countsByYear W19688634882016 @default.
• W1968863488 countsByYear W19688634882017 @default.
• W1968863488 countsByYear W19688634882018 @default.
• W1968863488 countsByYear W19688634882019 @default.
• W1968863488 countsByYear W19688634882020 @default.
• W1968863488 countsByYear W19688634882021 @default.
• W1968863488 countsByYear W19688634882022 @default.
• W1968863488 crossrefType "journal-article" @default.
• W1968863488 hasAuthorship W1968863488A5004319273 @default.
• W1968863488 hasAuthorship W1968863488A5008962287 @default.
• W1968863488 hasAuthorship W1968863488A5032406604 @default.
• W1968863488 hasAuthorship W1968863488A5062092073 @default.
• W1968863488 hasAuthorship W1968863488A5080182852 @default.

• next