Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968868669> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1968868669 endingPage "282" @default.
- W1968868669 startingPage "279" @default.
- W1968868669 abstract "A reliable and sensitive quantitative-PCR (Q-PCR) method using an in vitro culturing system for Cryptosporidium parvum has been developed and characterised. This method allows standardisation of an in vitro culturing system and its development for quantitative assessment using PCR. This system was assessed against an established counting method which is widely used to enumerate parasites, particularly following exposure to antiparasitic compounds. There are several sources of variability inherent in in vitro culturing systems which could result in an inaccurate final amount of DNA being detected per culture well. These can be summarised as cumulative effects due to variability in the in vitro system, and the DNA extraction and quantification method. Analysis of the variability in this in vitro culturing system using Q-PCR indicates that it offers higher sensitivity and specificity when compared with counting methods as well as providing a vast improvement in sample throughput and efficiency. Further to this, we have also determined a method for calculating the inhibitory concentration of anticryptosporidial compounds and present a comparison of this method with a counting method and published data. We conclude that this method of quantification could be used as a substitute for haemocytometer methods, particularly, and also antibody-based techniques." @default.
- W1968868669 created "2016-06-24" @default.
- W1968868669 creator A5004315768 @default.
- W1968868669 creator A5017979454 @default.
- W1968868669 creator A5047281553 @default.
- W1968868669 creator A5076918630 @default.
- W1968868669 creator A5086296060 @default.
- W1968868669 date "2002-05-01" @default.
- W1968868669 modified "2023-10-14" @default.
- W1968868669 title "The development of a real-time quantitative-PCR method for characterisation of a Cryptosporidium parvum in vitro culturing system and assessment of drug efficacy" @default.
- W1968868669 cites W1928134960 @default.
- W1968868669 cites W1994091239 @default.
- W1968868669 cites W2000997095 @default.
- W1968868669 cites W2040309911 @default.
- W1968868669 cites W2068935032 @default.
- W1968868669 cites W2070721758 @default.
- W1968868669 cites W2071221823 @default.
- W1968868669 cites W2094517427 @default.
- W1968868669 cites W2096905618 @default.
- W1968868669 cites W2107606382 @default.
- W1968868669 cites W2110768409 @default.
- W1968868669 cites W2129481909 @default.
- W1968868669 cites W2133223826 @default.
- W1968868669 cites W2134207220 @default.
- W1968868669 cites W2164578725 @default.
- W1968868669 cites W2167462261 @default.
- W1968868669 cites W2319800318 @default.
- W1968868669 cites W2412705065 @default.
- W1968868669 doi "https://doi.org/10.1016/s0166-6851(02)00041-5" @default.
- W1968868669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12034463" @default.
- W1968868669 hasPublicationYear "2002" @default.
- W1968868669 type Work @default.
- W1968868669 sameAs 1968868669 @default.
- W1968868669 citedByCount "38" @default.
- W1968868669 countsByYear W19688686692012 @default.
- W1968868669 countsByYear W19688686692013 @default.
- W1968868669 countsByYear W19688686692014 @default.
- W1968868669 countsByYear W19688686692019 @default.
- W1968868669 countsByYear W19688686692020 @default.
- W1968868669 countsByYear W19688686692022 @default.
- W1968868669 crossrefType "journal-article" @default.
- W1968868669 hasAuthorship W1968868669A5004315768 @default.
- W1968868669 hasAuthorship W1968868669A5017979454 @default.
- W1968868669 hasAuthorship W1968868669A5047281553 @default.
- W1968868669 hasAuthorship W1968868669A5076918630 @default.
- W1968868669 hasAuthorship W1968868669A5086296060 @default.
- W1968868669 hasConcept C104317684 @default.
- W1968868669 hasConcept C202751555 @default.
- W1968868669 hasConcept C2777795018 @default.
- W1968868669 hasConcept C2778877831 @default.
- W1968868669 hasConcept C2780035454 @default.
- W1968868669 hasConcept C48023723 @default.
- W1968868669 hasConcept C54355233 @default.
- W1968868669 hasConcept C61716771 @default.
- W1968868669 hasConcept C64903051 @default.
- W1968868669 hasConcept C70721500 @default.
- W1968868669 hasConcept C86803240 @default.
- W1968868669 hasConcept C89423630 @default.
- W1968868669 hasConcept C98274493 @default.
- W1968868669 hasConceptScore W1968868669C104317684 @default.
- W1968868669 hasConceptScore W1968868669C202751555 @default.
- W1968868669 hasConceptScore W1968868669C2777795018 @default.
- W1968868669 hasConceptScore W1968868669C2778877831 @default.
- W1968868669 hasConceptScore W1968868669C2780035454 @default.
- W1968868669 hasConceptScore W1968868669C48023723 @default.
- W1968868669 hasConceptScore W1968868669C54355233 @default.
- W1968868669 hasConceptScore W1968868669C61716771 @default.
- W1968868669 hasConceptScore W1968868669C64903051 @default.
- W1968868669 hasConceptScore W1968868669C70721500 @default.
- W1968868669 hasConceptScore W1968868669C86803240 @default.
- W1968868669 hasConceptScore W1968868669C89423630 @default.
- W1968868669 hasConceptScore W1968868669C98274493 @default.
- W1968868669 hasIssue "2" @default.
- W1968868669 hasLocation W19688686691 @default.
- W1968868669 hasLocation W19688686692 @default.
- W1968868669 hasOpenAccess W1968868669 @default.
- W1968868669 hasPrimaryLocation W19688686691 @default.
- W1968868669 hasRelatedWork W1550230601 @default.
- W1968868669 hasRelatedWork W1585256759 @default.
- W1968868669 hasRelatedWork W1996201574 @default.
- W1968868669 hasRelatedWork W2037633791 @default.
- W1968868669 hasRelatedWork W2042043248 @default.
- W1968868669 hasRelatedWork W2370052552 @default.
- W1968868669 hasRelatedWork W2371100938 @default.
- W1968868669 hasRelatedWork W2376961485 @default.
- W1968868669 hasRelatedWork W2419234509 @default.
- W1968868669 hasRelatedWork W3147759110 @default.
- W1968868669 hasVolume "121" @default.
- W1968868669 isParatext "false" @default.
- W1968868669 isRetracted "false" @default.
- W1968868669 magId "1968868669" @default.
- W1968868669 workType "article" @default.